Genome-wide regulation of innate immunity by juvenile hormone and 20-hydroxyecdysone in the Bombyx fat body.
Genome-wide regulation of innate immunity by juvenile hormone and 20-hydroxyecdysone in the Bombyx fat body.
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家蚕脂肪体内保幼激素和 20-羟基蜕皮酮对先天免疫的全基因组调节
DOI：
10.1186/1471-2164-11-549
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发表时间：
2010-10-09
期刊：
影响因子：
4.4
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Tian L;Guo E;Diao Y;Zhou S;Peng Q;Cao Y;Ling E;Li S
背景
保幼激素（JH）和20-羟基蜕皮激素（20 E）可以影响昆虫的先天免疫，但这两种激素对昆虫先天免疫的发育调控机制尚不清楚。在家蚕中，JH和20 E在幼虫最后蜕皮期间（4 M）水平较高，但在5龄摄食期（5 F）不存在，而在预蛹期（PP）JH水平较低，20 E水平较高。脂肪体产生体液反应分子，因此被认为是参与先天免疫的主要器官。
结果
从4 M、5 F和PP分离的脂肪体的全基因组微阵列分析揭示了大量差异表达的基因。最值得注意的是，6个抗菌肽（AMP）基因在4 M时相对于PP上调，这表明PGEX先天免疫是由两种激素发育调节的。首先，JH处理显著增加AMP mRNA水平和活性。此外，20 E处理表现出对AMP mRNA水平和活性的抑制作用，20 E受体EcR-USP的RNA干扰具有与20 E处理相反的作用。
结论
两者合计，我们表明，JH作为一种免疫激活剂，而20 E抑制先天性免疫在脂肪体发育过程中的脂肪。
Background
Insect innate immunity can be affected by juvenile hormone (JH) and 20-hydroxyecdysone (20E), but how innate immunity is developmentally regulated by these two hormones in insects has not yet been elucidated. In the silkworm, Bombyx mori, JH and 20E levels are high during the final larval molt (4 M) but absent during the feeding stage of 5th instar (5 F), while JH level is low and 20E level is high during the prepupal stage (PP). Fat body produces humoral response molecules and hence is considered as the major organ involved in innate immunity.
Results
A genome-wide microarray analysis of Bombyx fat body isolated from 4 M, 5 F and PP uncovered a large number of differentially-expressed genes. Most notably, 6 antimicrobial peptide (AMP) genes were up-regulated at 4 M versus PP suggesting that Bombyx innate immunity is developmentally regulated by the two hormones. First, JH treatment dramatically increased AMP mRNA levels and activities. Furthermore, 20E treatment exhibited inhibitory effects on AMP mRNA levels and activities, and RNA interference of the 20E receptor EcR-USP had the opposite effects to 20E treatment.
Conclusion
Taken together, we demonstrate that JH acts as an immune-activator while 20E inhibits innate immunity in the fat body during Bombyx postembryonic development.
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影响因子：
2.6
作者：
Muramatsu, Daisuke;Kinjoh, Terunori;Hiruma, Kiyoshi
通讯作者：
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DOI：
10.1073/pnas.0500954102
发表时间：
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Riddiford, LM;Hiruma, K;Nelson, CA
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