Fos proteins are not prerequisite for osmotic induction of vasopressin transcription in supraoptic nucleus of rats.
Fos proteins are not prerequisite for osmotic induction of vasopressin transcription in supraoptic nucleus of rats.
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DOI：
10.1016/j.neulet.2010.09.030
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发表时间：
2010-12-03
影响因子：
2.5
通讯作者：
Aguilera G
中科院分区：
文献类型：
--
作者：
Arima H;Baler R;Aguilera G
渗透压刺激可诱导视上核(SON)Fos家族成员的表达，但其诱导蛋白产物是否参与精氨酸加压素(AVP)基因转录的调控尚不清楚。在本研究中，我们研究了急性渗透刺激后SON中不同Fos家族成员AVP基因转录和表达变化之间的体内相关性。结果表明，注射高渗盐水后15min，AVP转录高峰(内含子原位杂交)先于Fos蛋白表达，30min可检测到AVP转录高峰，120min达高峰。凝胶迁移率改变分析表明，表达的Fos蛋白结合在AVP启动子的AP-1/Cre样复合位点上。这些结果表明，急性渗透刺激诱导的SON中的Fos蛋白可能通过与AVP启动子结合而影响AVP基因的转录，但不是诱导AVP基因转录的先决条件。
While it is well known that osmotic stimulation induces the expression of Fos family members in the supraoptic nucleus (SON), it is unclear whether the induced protein products are involved in the regulation of the gene transcription of arginine vasopressin (AVP). In the present study, we examined the in vivo correlation between changes in AVP gene transcription and expression of the various Fos family members in the SON after acute osmotic stimuli. The data demonstrated that the peak of AVP transcription (measured by intronic in situ hybridization) observed 15 min after an injection of hypertonic saline preceded the expression of Fos proteins, which became detectable at 30 min and peaked at 120 min. Electrophoretic mobility shift assay showed that the expressed Fos proteins bound to the composite AP-1/CRE-like site in the AVP promoter. These data suggest that Fos proteins in the SON induced by acute osmotic stimuli could affect AVP gene transcription by binding to the AVP promoter, but that they are not prerequisite for the induction of AVP gene transcription.
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