A distinct assembly pathway of the human 39S late pre-mitoribosome.
A distinct assembly pathway of the human 39S late pre-mitoribosome.
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DOI：
10.1038/s41467-021-24818-x
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发表时间：
2021-07-27
影响因子：
16.6
通讯作者：
Beckmann R
中科院分区：
文献类型：
--
作者：
Cheng J;Berninghausen O;Beckmann R
有丝分裂体的组装在很大程度上是神秘的，涉及许多组装因子。关于它们的功能和前核糖体中间体的结构转变知之甚少。在这里，我们解决了冷冻电镜结构的人39 S大亚基前核糖体，代表五个不同的晚期状态。除了用作亲和纯化诱饵的MALSU 1复合物外，我们还鉴定了几种组装因子，包括DDX 28解旋酶、MRM 3、GTPBP 10和NSUN 4-mTERF 4复合物，所有这些因子均使16 S rRNA保持未成熟构象。晚期转换主要涉及rRNA结构域IV和V，它们形成39 S亚基的中央突起、亚基间侧和肽基转移酶中心。出乎意料的是，我们在核糖体E位点发现了脱酰化的tRNA，这表明它在39 S组装中起作用。总之，我们的研究提供了一个建筑库存的独特的晚期组装阶段的人39 S线粒体。线粒体的组装需要许多专门因素的帮助。在这里，人类39 S晚期组装中间体的结构确定了几个组装因子，这些组装因子使16 S rRNA保持不成熟的构象，并揭示了核糖体E位点的脱酰tRNA，表明在39 S组装中的作用。
Assembly of the mitoribosome is largely enigmatic and involves numerous assembly factors. Little is known about their function and the architectural transitions of the pre-ribosomal intermediates. Here, we solve cryo-EM structures of the human 39S large subunit pre-ribosomes, representing five distinct late states. Besides the MALSU1 complex used as bait for affinity purification, we identify several assembly factors, including the DDX28 helicase, MRM3, GTPBP10 and the NSUN4-mTERF4 complex, all of which keep the 16S rRNA in immature conformations. The late transitions mainly involve rRNA domains IV and V, which form the central protuberance, the intersubunit side and the peptidyltransferase center of the 39S subunit. Unexpectedly, we find deacylated tRNA in the ribosomal E-site, suggesting a role in 39S assembly. Taken together, our study provides an architectural inventory of the distinct late assembly phase of the human 39S mitoribosome. Assembly of the mitoribosome requires assistance from numerous specialized factors. Here, structures of the human 39S late assembly intermediates identify several assembly factors which keep the 16S rRNA in immature conformations, and reveal deacylated tRNA in the ribosomal E-site, suggesting a role in 39S assembly.
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影响因子：
29
作者：
Camara, Yolanda;Asin-Cayuela, Jorge;Larsson, Nils-Goran
通讯作者：
Larsson, Nils-Goran
DOI：
10.1126/science.aaa1193
发表时间：
2015-04-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Amunts A;Brown A;Toots J;Scheres SHW;Ramakrishnan V
通讯作者：
Ramakrishnan V
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
DOI：
10.1107/s0907444909042073
发表时间：
2010-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Chen VB;Arendall WB 3rd;Headd JJ;Keedy DA;Immormino RM;Kapral GJ;Murray LW;Richardson JS;Richardson DC
通讯作者：
Richardson DC
影响因子：
56.9
作者：
Amunts, Alexey;Brown, Alan;Ramakrishnan, V.
通讯作者：
Ramakrishnan, V.