Sequencing of the complete mitochondrial genome of the common raven Corvus corax (Aves: Corvidae) confirms mitogenome-wide deep lineages and a paraphyletic relationship with the Chihuahuan raven C. cryptoleucus.
Sequencing of the complete mitochondrial genome of the common raven Corvus corax (Aves: Corvidae) confirms mitogenome-wide deep lineages and a paraphyletic relationship with the Chihuahuan raven C. cryptoleucus.
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DOI：
10.1371/journal.pone.0187316
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Anmarkrud JA
中科院分区：
文献类型：
--
作者：
Johnsen A;Kearns AM;Omland KE;Anmarkrud JA
先前基于单个线粒体标记的研究表明，普通乌鸦（Corvus corax）由两个高度分化的谱系组成，假设在二次接触时经历了物种形成逆转。此外，基于线粒体DNA (mtDNA)，普通乌鸦与奇瓦瓦渡鸦（C. cryptoleucus）有亲缘关系。在这里，我们通过对来自北半球的12只常见乌鸦的全线粒体基因组测序，以及3只吉娃娃乌鸦和1只近亲棕颈乌鸦（C. ruficollis）的全线粒体基因组测序，探讨了mtDNA缺失的原因。我们的乌鸦有丝分裂基因组长度在16925-16928 bp之间。GC含量从43.3%到43.8%不等，13个蛋白质编码基因、2个rrna和22个trna符合标准的鸟类线粒体排列。两种常见乌鸦枝的总体差异为3%（包括蛋白质编码基因、rnas和控制区在内的16个区域的差异范围为0.3-5.8%）。利用全有丝分裂基因组构建的系统发育学恢复了之前发现的加利福尼亚渡鸦和奇瓦瓦渡鸦之间的线粒体姐妹关系（总差异为1.1%），这加强了普通渡鸦mtDNA缺失是由先前截然不同的全北极和加利福尼亚谱系的物种形成逆转造成的假设。
Previous studies based on single mitochondrial markers have shown that the common raven (Corvus corax) consists of two highly diverged lineages that are hypothesised to have undergone speciation reversal upon secondary contact. Furthermore, common ravens are paraphyletic with respect to the Chihuahuan raven (C. cryptoleucus) based on mitochondrial DNA (mtDNA). Here we explore the causes of mtDNA paraphyly by sequencing whole mitochondrial genomes of 12 common ravens from across the Northern Hemisphere, in addition to three Chihuahuan ravens and one closely related brown-necked raven (C. ruficollis) using a long-range PCR protocol. Our raven mitogenomes ranged between 16925–16928 bp in length. GC content varied from 43.3% to 43.8% and the 13 protein coding genes, two rRNAs and 22 tRNAs followed a standard avian mitochondrial arrangement. The overall divergence between the two common raven clades was 3% (range 0.3–5.8% in 16 regions including the protein coding genes, rRNAs and the control region). Phylogenies constructed from whole mitogenomes recovered the previously found mitochondrial sister relationship between the common raven California clade and the Chihuahuan raven (overall divergence 1.1%), which strengthens the hypothesis that mtDNA paraphyly in the common raven results from speciation reversal of previously distinct Holarctic and California lineages.
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影响因子：
4.3
作者：
Bragg LM;Stone G;Butler MK;Hugenholtz P;Tyson GW
通讯作者：
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