Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription.
Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription.
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DOI：
10.1002/eji.201445295
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发表时间：
2015-04
影响因子：
5.4
通讯作者：
Radbruch, Andreas
中科院分区：
文献类型：
--
作者：
Alp, Oezen Sercan;Durlanik, Sibel;Schulz, Daniel;McGrath, Mairi;Gruen, Joachim R.;Bardua, Marcus;Ikuta, Koichi;Sgouroudis, Evridiki;Riedel, Rene;Zehentmeier, Sandra;Hauser, Anja E.;Tsuneto, Motokazu;Melchers, Fritz;Tokoyoda, Koji;Chang, Hyun-Dong;Thiel, Andreas;Radbruch, Andreas
据信，记忆性CD 8 + T细胞通过稳态增殖维持在次级淋巴组织、外周组织和BM中。他们的生存已被证明是依赖于IL-7，但目前还不清楚他们在哪里获得it. In这里，我们表明，在小鼠骨髓，记忆CD 8 + T细胞单独共定位与IL-7+网状基质细胞。T细胞在整体转录方面处于静止状态，并且不表达活化标志物，例如4-1BB（CD 137）、IL-2或IFN-γ，尽管在约30%的细胞上表达CD 69。骨髓中95%的记忆性CD 8 + T细胞处于细胞周期的G 0期，不表达Ki-67。碘化丙啶染色显示细胞周期处于S/M/G2期者少于1%。虽然以前的出版物估计的程度上的CD 8+记忆T细胞的增殖的基础上的BrdU掺入，我们在这里表明，BrdU本身诱导CD 8+记忆T细胞的增殖。综上所述，本结果表明，CD 8+记忆T细胞作为静息细胞在BM中维持在专用的小生境中，其存活取决于IL-7受体信号传导。
It is believed that memory CD8+ T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8+ T cells individually colocalize with IL-7+ reticular stromal cells. The T cells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-γ, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8+ T cells in BM are in G0 phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G2 of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8+ memory T cells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8+ memory T cells. Taken together, the present results suggest that CD8+ memory T cells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling.
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影响因子：
4.4
作者：
Hauser, AE;Debes, GF;Manz, RA
通讯作者：
Manz, RA
DOI：
10.1084/jem.20030735
发表时间：
2003-12-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kondrack RM;Harbertson J;Tan JT;McBreen ME;Surh CD;Bradley LM
通讯作者：
Bradley LM
影响因子：
4.4
作者：
Hara, Takahiro;Shitara, Soichiro;Ikuta, Koichi
通讯作者：
Ikuta, Koichi
影响因子：
64.8
作者：
Jiang, Xiaodong;Clark, Rachael A.;Liu, Luzheng;Wagers, Amy J.;Fuhlbrigge, Robert C.;Kupper, Thomas S.
通讯作者：
Kupper, Thomas S.
DOI：
10.1073/pnas.0706040104
发表时间：
2007-08-14
影响因子：
11.1
作者：
Heffner, Maike;Fearon, Douglas T.
通讯作者：
Fearon, Douglas T.