Emerging citrus diseases in Europe caused by species of Diaporthe.
Emerging citrus diseases in Europe caused by species of Diaporthe.
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DOI：
10.5598/imafungus.2017.08.02.07
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发表时间：
2017-12
期刊：
影响因子：
5.4
通讯作者：
Crous PW
中科院分区：
文献类型：
--
作者：
Guarnaccia V;Crous PW
Diaporthe 物种被认为是多种植物宿主上重要的植物病原体、腐生菌和内生菌。有几个物种在柑橘类水果中广为人知，它们可以作为采收前或采后感染的病原体，例如水果上的枯萎病、黑素病和茎端腐烂病。在这项研究中，我们探讨了欧洲果园、苗圃和花园中与柑橘属及近缘属相关的黄霉属物种的发生、多样性和致病性。 2015 年和 2016 年在希腊、意大利、马耳他、葡萄牙和西班牙进行了调查。从有症状的小枝、树枝和树干中总共分离出 79 株 Diaporthe 菌株。基于五个基因组位点（ITS、tef1、cal、his3 和 tube2）建立了多位点系统发育，并确定了分离株的形态特征。对具有代表性分离株的柠檬、酸橙和橙子植物进行了初步致病性测试。最常见的分离物种是 D. foeniculina 和 D. baccae，而仅收集了 4 个 D. novem 分离株。两种新的 Diaporthe 物种，此处描述为 D. limonicola 和 D. melitensis spp。十一月被发现与柠檬植物的一种新的毁灭性顶枯病有关。此外，一簇不育的Diaporthe分离株被重新命名为D. infertilis。致病性测试表明，大多数柑橘属物种对酒红色果蝇、茴香果蝇和新果果蝇敏感。此外，D. limonicola 和 D. melitensis 会引起严重的溃疡病，影响所有测试的柑橘品种。这项研究是欧洲首次报道柑橘上的 D. baccae 和 D. novem，也是欧洲首次检测到柑橘新的 Diaporthe 溃疡病。然而，没有发现柑橘木虱的分离株。该研究提高了我们对与柑橘类植物几种疾病症状相关的物种的了解，并为有效的疾病管理提供了有用的信息。
Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on citrus, either as agents of pre- or post-harvest infections, such as dieback, melanose and stem-end rot on fruit. In this study we explored the occurrence, diversity and pathogenicity of Diaporthe species associated with Citrus and allied genera in European orchards, nurseries, and gardens. Surveys were carried out during 2015 and 2016 in Greece, Italy, Malta, Portugal, and Spain. A total of 79 Diaporthe strains were isolated from symptomatic twigs, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates determined. Preliminary pathogenicity tests were performed on lemon, lime, and orange plants with representative isolates. The most commonly isolated species were D. foeniculina and D. baccae, while only four isolates of D. novem were collected. Two new Diaporthe species, described here as D. limonicola and D. melitensis spp. nov. were found associated with a new devastating dieback disease of lemon plants. Furthermore, one cluster of sterile Diaporthe isolates was renamed as D. infertilis. Pathogenicity tests revealed most of the Citrus species as susceptible to D. baccae, D. foeniculina, and D. novem. Moreover, D. limonicola and D. melitensis caused serious cankers affecting all the Citrus species tested. This study is the first report of D. baccae and D. novem on citrus in Europe, and the first detection of a new Diaporthe canker disease of citrus in Europe. However, no isolates of D. citri were found. The study improves our understanding of the species associated with several disease symptoms on citrus plants, and provides useful information for effective disease management.
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影响因子：
4.5
作者：
Diaz, Gonzalo A.;Latorre, Bernardo A.;Zoffoli, Juan P.
通讯作者：
Zoffoli, Juan P.
影响因子：
2.8
作者：
Dai, JQ;Krohn, K;Rheinheimer, J
通讯作者：
Rheinheimer, J
影响因子：
4.4
作者：
GLASS, NL;DONALDSON, GC
通讯作者：
DONALDSON, GC
影响因子：
1.5
作者：
Aiello, Dalia;Carrieri, Raffaele;Polizzi, Giancarlo
通讯作者：
Polizzi, Giancarlo
影响因子：
9.1
作者：
Crous PW;Summerell BA;Swart L;Denman S;Taylor JE;Bezuidenhout CM;Palm ME;Marincowitz S;Groenewald JZ
通讯作者：
Groenewald JZ