Phylogenomic and functional characterization of an evolutionary conserved cytochrome P450-based insecticide detoxification mechanism in bees.
Phylogenomic and functional characterization of an evolutionary conserved cytochrome P450-based insecticide detoxification mechanism in bees.
复制标题
DOI：
10.1073/pnas.2205850119
复制
发表时间：
2022-06-28
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
--
作者：
蜜蜂传粉者农药风险评估是农药注册的监管要求，主要基于为替代物种（如西方蜜蜂）收集的实验数据。最近，CYP9Q3，一种蜜蜂细胞色素P450酶，已被证明能有效地解毒某些杀虫剂，如丁烯二酰氟吡呋酮和烟碱类噻虫啉。在这里，我们分析了75种蜜蜂的基因组数据，并通过重组表达26个CYP9Q3推定的功能直系同源物证明，这种解毒原理是一种进化保守的机制，在蜜蜂家族。我们的毒理基因组学方法有可能为无法进行急性毒性测试的非管理蜜蜂物种的农药风险评估提供信息。评估杀虫剂对蜜蜂风险的监管程序在很大程度上依赖于使用蜜蜂作为其他蜜蜂物种的模型。然而，使用A.在农药风险评估中，意大利蜜蜂作为其他蜜蜂和非蜜蜂的替代品一直受到质疑。与此相关的研究，最近的工作A。意大利蜜蜂属植物已经表明，属于CYP9Q亚家族的特异性P450酶通过有效地使某些杀虫剂化学型解毒而在该物种中作为杀虫剂敏感性的至关重要的决定因素。然而，这些酶的功能性直系同源物在蜜蜂多样性中的保守程度尚不清楚。在这里，我们使用了一种新的基因组方法来鉴定75个蜜蜂物种中超过100个推定的CYP9Q功能直系同源物，包括所有主要的蜜蜂家族。26 P450从20个代表性的蜜蜂物种的功能分析表明，P450介导的解毒某些系统性杀虫剂，包括烟碱类噻虫啉和butenelaflupyradifurone，是保守的所有主要蜜蜂传粉家庭。然而，我们的分析也表明，CYP9Q相关基因并不普遍的所有蜜蜂物种，与一些Megachilidae物种缺乏这样的基因。因此，我们的研究结果揭示了在所有主要蜜蜂家族中代谢某些杀虫剂的进化保守能力，同时确定了少数蜜蜂物种，这种功能可能已经丢失。此外，他们说明了潜在的毒理基因组学方法，通过预测蜜蜂授粉物种分解合成杀虫剂的能力，为非管理蜜蜂物种的农药风险评估提供信息。
Bee pollinator pesticide risk assessment is a regulatory requirement for pesticide registration and is largely based on experimental data collected for surrogate species such as the western honeybee. Recently, CYP9Q3, a honeybee cytochrome P450 enzyme, has been shown to efficiently detoxify certain insecticides such as the butenolide flupyradifurone and the neonicotinoid thiacloprid. Here we analyzed genomic data for 75 bee species and demonstrated by the recombinant expression of 26 CYP9Q3 putative functional orthologs that this detoxification principle is an evolutionary conserved mechanism across bee families. Our toxicogenomics approach has the potential to inform pesticide risk assessment for nonmanaged bee species that are not accessible for acute toxicity testing. The regulatory process for assessing the risks of pesticides to bees relies heavily on the use of the honeybee, Apis mellifera, as a model for other bee species. However, the validity of using A. mellifera as a surrogate for other Apis and non-Apis bees in pesticide risk assessment has been questioned. Related to this line of research, recent work on A. mellifera has shown that specific P450 enzymes belonging to the CYP9Q subfamily act as critically important determinants of insecticide sensitivity in this species by efficiently detoxifying certain insecticide chemotypes. However, the extent to which the presence of functional orthologs of these enzymes is conserved across the diversity of bees is unclear. Here we used a phylogenomic approach to identify > 100 putative CYP9Q functional orthologs across 75 bee species encompassing all major bee families. Functional analysis of 26 P450s from 20 representative bee species revealed that P450-mediated detoxification of certain systemic insecticides, including the neonicotinoid thiacloprid and the butenolide flupyradifurone, is conserved across all major bee pollinator families. However, our analyses also reveal that CYP9Q-related genes are not universal to all bee species, with some Megachilidae species lacking such genes. Thus, our results reveal an evolutionary conserved capacity to metabolize certain insecticides across all major bee families while identifying a small number of bee species where this function may have been lost. Furthermore, they illustrate the potential of a toxicogenomic approach to inform pesticide risk assessment for nonmanaged bee species by predicting the capability of bee pollinator species to break down synthetic insecticides.
登录
查看更多内容
影响因子：
14.9
作者：
Blum M;Chang HY;Chuguransky S;Grego T;Kandasaamy S;Mitchell A;Nuka G;Paysan-Lafosse T;Qureshi M;Raj S;Richardson L;Salazar GA;Williams L;Bork P;Bridge A;Gough J;Haft DH;Letunic I;Marchler-Bauer A;Mi H;Natale DA;Necci M;Orengo CA;Pandurangan AP;Rivoire C;Sigrist CJA;Sillitoe I;Thanki N;Thomas PD;Tosatto SCE;Wu CH;Bateman A;Finn RD
通讯作者：
Finn RD
影响因子：
--
作者：
Connolly CN
通讯作者：
Connolly CN
影响因子：
14.8
作者：
通讯作者：
--
影响因子：
5.3
作者：
Biddinger, David J.;Rajotte, Edwin G.
通讯作者：
Rajotte, Edwin G.
影响因子：
11.8
作者：
Haas, Julian;Nauen, Ralf
通讯作者：
Nauen, Ralf