Sulfuricystis multivorans gen. nov., sp. nov. and Sulfuricystis thermophila sp. nov., facultatively autotropic sulfur-oxidizing bacteria isolated from a hot spring, and emended description of the genus Rugosibacter
Sulfuricystis multivorans gen. nov., sp. nov. and Sulfuricystis thermophila sp. nov., facultatively autotropic sulfur-oxidizing bacteria isolated from a hot spring, and emended description of the genus Rugosibacter
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多食硫囊藻属
DOI：
10.1007/s00203-022-03186-0
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发表时间：
2022
影响因子：
2.8
通讯作者：
Fukui Manabu
中科院分区：
文献类型：
--
作者：
Kojima Hisaya;Watanabe Miho;Miyata Naoyuki;Fukui Manabu
菌株 J5BT 和 M52Ta 是从温泉微生物垫中分离出来的兼性自养硫氧化细菌。它们在之前的研究中被分离并部分表征为使用硝酸盐作为电子受体的兼性厌氧菌。在这项研究中，进行了额外的表征以确定它们的分类地位。在两种菌株中，主要细胞脂肪酸为 C16:1（C16:1ω7c 和/或 C16:1ω6c）和 C16:0。它们的化能自养生长得到硫代硫酸盐和元素硫的支持。他们使用一些有机酸作为生长基质。它们的 16S rRNA 基因序列表明与甾菌科物种的序列同一性最高，但同一性为 95% 或更低。系统发育分析表明这些菌株不属于任何现有属。菌株J5BT和M52T之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为87.93%和34.3%。根据表型和基因组特征，Sulfuricystis multivoransgen。十一月sp。 11月，和嗜热硫囊菌。十一月提出的菌株分别为 J5BT 和 M52T。还提出了对 Rugosibacteris 属的修订描述，将其重新分类为甾类细菌科 (Sterolibacteriaceae)。
Strains J5BTand M52Tare facultatively autotrophic sulfur-oxidizing bacteria isolated from a microbial mat from a hot spring. They were isolated and partially characterized in previous studies, as facultative anaerobes which use nitrate as electron acceptor. In this study, additional characterizations were made to determine their taxonomic status. In both strains, major cellular fatty acids were C16:1(C16:1ω7c and/or C16:1ω6c) and C16:0. Their chemolithoautotrophic growth was supported by thiosulfate and elemental sulfur. They used some organic acids as growth substrates. Their 16S rRNA gene sequences indicated the highest sequence identities to species in the familySterolibacteriaceae, but the identities were 95% or lower. Phylogenetic analysis indicated that these strains do not belong to any existing genera. Values of average nucleotide identity and digital DNA–DNA hybridization between strains J5BTand M52Twere 87.93% and 34.3%, respectively. On the basis of phenotypic and genomic characteristics,Sulfuricystis multivoransgen. nov. sp. nov., andSulfuricystis thermophilasp. nov. are proposed, with type strains of J5BTand M52T, respectively. An emended description of the genusRugosibacteris also proposed, for its reclassification to the familySterolibacteriaceae.
DOI：
10.1099/ijsem.0.001622
发表时间：
2017-02-01
影响因子：
2.8
作者：
Corteselli, Elizabeth M.;Aitken, Michael D.;Singleton, David R.
通讯作者：
Singleton, David R.
Eztree：一种自动化管道，用于鉴定系统发育基因基因并推断出未培养的原核基因组之间的进化关系。
影响因子：
4.4
作者：
Wu YW
通讯作者：
Wu YW
DOI：
10.1099/ijs.0.055046-0
发表时间：
2014-01-01
影响因子：
2.8
作者：
Doronina, Nina V.;Kaparullina, Elena N.;Trotsenko, Yuri A.
通讯作者：
Trotsenko, Yuri A.