Redistribution of FLAgellar Member 8 during the trypanosome life cycle: Consequences for cell fate prediction.
Redistribution of FLAgellar Member 8 during the trypanosome life cycle: Consequences for cell fate prediction.
复制标题
锥虫生命周期中FLAgellar成员8的重新分布：细胞命运预测的结果。
DOI：
10.1111/cmi.13347
复制
发表时间：
2021-09
影响因子：
3.4
通讯作者：
Rotureau B
中科院分区：
文献类型：
--
作者：
Calvo-Álvarez E;Bonnefoy S;Salles A;Benson FE;McKean PG;Bastin P;Rotureau B
非洲锥虫的单鞭毛在寄生虫发育的多个方面都是必不可少的。鞭毛成员8蛋白（FLAM8），定位于培养的布氏锥虫的鞭毛顶端，被确定为控制鞭毛长度的锁定事件的标记。在这里，我们研究了FLAM8是否也能反映其他寄生虫周期阶段的鞭毛成熟状态。我们观察到，在哺乳动物感染形式中，FLAM8分布沿着整个鞭毛细胞骨架延伸。然后，在分化成早期昆虫形态的过程中，FLAM8迅速集中到远端，说明了现有鞭毛的重塑。在采采心中，在不对称分裂过程中，FLAM8进一步定位于新鞭毛的整个长度。引人注目的是，在采采中肠和唾液腺中分裂的寄生虫中，新鞭毛中FLAM8的数量和分布可以预测子细胞的命运。我们提出并讨论了FLAM8如何被认为是锥虫鞭毛阶段和成熟状态的元标记物。鞭毛成员8蛋白（FLAgellar Member 8 protein, FLAM8）被鉴定为控制非洲锥虫鞭毛长度锁定事件的标记。在这里，我们发现在布鲁氏体的哺乳动物感染形式（ST）中，FLAM8沿着整个鞭毛细胞骨架延伸，并在分化到昆虫原循环阶段（PCF）时集中到尖端。在采采中肠分裂的寄生虫中，新鞭毛中FLAM8的数量和分布预示着子细胞的命运，要么是走向另一轮增殖，要么是走向鞭毛伸长和下一轮细胞分化（LT和DE）。总之，FLAM8可以被认为是锥虫鞭毛阶段和成熟状态的元标记物。
The single flagellum of African trypanosomes is essential in multiple aspects of the parasites' development. The FLAgellar Member 8 protein (FLAM8), localised to the tip of the flagellum in cultured insect forms of Trypanosoma brucei, was identified as a marker of the locking event that controls flagellum length. Here, we investigated whether FLAM8 could also reflect the flagellum maturation state in other parasite cycle stages. We observed that FLAM8 distribution extended along the entire flagellar cytoskeleton in mammalian‐infective forms. Then, a rapid FLAM8 concentration to the distal tip occurs during differentiation into early insect forms, illustrating the remodelling of an existing flagellum. In the tsetse cardia, FLAM8 further localises to the entire length of the new flagellum during an asymmetric division. Strikingly, in parasites dividing in the tsetse midgut and in the salivary glands, the amount and distribution of FLAM8 in the new flagellum were seen to predict the daughter cell fate. We propose and discuss how FLAM8 could be considered a meta‐marker of the flagellum stage and maturation state in trypanosomes. FLAgellar Member 8 protein (FLAM8) was identified as a marker of the locking event that controls flagellum length in African trypanosomes. Here, we show that FLAM8 extends along the entire flagellar cytoskeleton in mammalian infective forms (ST) of T. brucei and concentrates to the tip during differentiation into the insect procyclic stage (PCF). In parasites dividing in the tsetse midgut, the amount and distribution of FLAM8 in the new flagellum predicts the daughter cell fate, either towards another round of proliferation or towards flagellum elongation and next cell differentiation (LT and DE). In total, FLAM8 could be considered as a meta‐marker of the flagellum stage and maturation state in trypanosomes.
登录
查看更多内容
影响因子：
11.4
作者：
Kohl, L;Robinson, D;Bastin, P
通讯作者：
Bastin, P
影响因子：
3.6
作者：
Abeywickrema, Movin;Vachova, Hana;Varga, Vladimir
通讯作者：
Varga, Vladimir
影响因子：
1.5
作者：
Bringaud, F;Robinson, DR;Baltz, T
通讯作者：
Baltz, T
影响因子：
1.5
作者：
Burkard, Gabriela;Fragoso, Cristina M.;Roditi, Isabel
通讯作者：
Roditi, Isabel
影响因子：
1.5
作者：
Kelly, Steven;Reed, Jenny;Kramer, Susanne;Ellis, Louise;Webb, Helena;Sunter, Jack;Salje, Jeanne;Marinsek, Nina;Gull, Keith;Wickstead, Bill;Carrington, Mark
通讯作者：
Carrington, Mark