Metabolomics of AS-5 RBC supernatants following routine storage.
Metabolomics of AS-5 RBC supernatants following routine storage.
复制标题
常规存储后AS-5 RBC上清液的代谢组学。
DOI：
10.1111/vox.12193
复制
发表时间：
2015-02
期刊：
影响因子：
2.7
通讯作者：
Banerjee A
中科院分区：
文献类型：
--
作者：
D'Alessandro A;Hansen KC;Silliman CC;Moore EE;Kelher M;Banerjee A
由于回顾性的临床证据和实验室结果，输注储存的红细胞(RBC)的安全性和有效性一直存在争议，表明输注储存超过14天的RBC单位后发病率增加和死亡率之间存在潜在的相关性。我们假设储存在Optisol添加剂溶液-5中的红细胞在储存的红细胞上清液中产生独特的代谢组学特征。从5名健康献血者中抽取全血，制成红细胞单位，通过过滤去除保存前的白细胞。在第1天和第42天采集样本，取出细胞，进行基于质谱学的代谢组学研究。结果证实了42天前RBC能量代谢的进行性损害，间接标记物是谷胱甘肽和NADPH稳态平行改变。此外，氧化的促炎脂质在储存结束时积累。从代谢组学的角度来看，来自储存的红细胞的上清液可能对输血受体来说是一种负担。
The safety and efficacy of stored red blood cells (RBCs) transfusion has been long debated due to retrospective clinical evidence and laboratory results, indicating a potential correlation between increased morbidity and mortality following transfusion of RBC units stored longer than 14 days. We hypothesize that storage in Optisol additive solution-5 leads to a unique metabolomics profile in the supernatant of stored RBCs. Whole blood was drawn from five healthy donors, RBC units were manufactured, and prestorage leucoreduced by filtration. Samples were taken on days 1 and 42, the cells removed, and mass spectrometry-based metabolomics was performed. The results confirmed the progressive impairment of RBC energy metabolism by day 42 with indirect markers of a parallel alteration of glutathione and NADPH homeostasis. Moreover, oxidized pro-inflammatory lipids accumulated by the end of storage. The supernatants from stored RBCs may represent a burden to the transfused recipients from a metabolomics standpoint.
登录
查看更多内容
影响因子：
2.9
作者：
Burger, Patrick;Korsten, Herbert;Verhoeven, Arthur J.
通讯作者：
Verhoeven, Arthur J.
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
2.9
作者：
Lelubre, Christophe;Piagnerelli, Michael;Vincent, Jean-Louis
通讯作者：
Vincent, Jean-Louis
影响因子：
4.8
作者：
Koivunen, Peppi;Hirsila, Maija;Myllyharju, Johanna
通讯作者：
Myllyharju, Johanna
影响因子：
3.4
作者：
Bicalho, Beatriz;Holovati, Jelena L.;Acker, Jason P.
通讯作者：
Acker, Jason P.