Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics.
Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics.
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DOI：
10.3389/fcimb.2012.00002
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发表时间：
2012
影响因子：
5.7
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
He Y
布鲁氏菌是一种革兰氏阴性、兼性胞内细菌，可引起人类和各种动物的人畜共患布鲁氏菌病。在10种布氏菌中，B. melitensis，B. abortus，牛种B. suis和B.犬对人类有致病性。在过去的十年中，布鲁氏菌的致病机制和宿主免疫已被广泛研究，利用先进的系统生物学和生物信息学方法。本文综述了组学（包括基因组学、转录组学和蛋白质组学）和生物信息学技术在布鲁氏菌发病机制、宿主免疫应答和疫苗靶点分析中的应用。基于30多个测序的布鲁氏菌基因组，比较基因组学能够识别布鲁氏菌菌株之间的基因变异，有助于解释布鲁氏菌物种之间的宿主特异性和毒力差异。不同的转录组学和蛋白质组学基因表达研究已被进行，以分析在不同的实验室条件下的野生型布鲁氏菌菌株和突变株的基因表达谱。宿主对强毒或减毒布鲁氏菌菌株感染的反应的高通量组学分析集中在小鼠和牛巨噬细胞、牛滋养层细胞、小鼠和野猪脾细胞以及公羊血沉棕黄层的反应。采用高通量血清抗体筛选技术分析了人和公羊对布鲁氏菌感染的血清反应差异。Vaxign反向疫苗学已用于预测许多布鲁氏菌疫苗靶点。利用先进的文献挖掘方法，已经确定了180多个布鲁氏菌毒力因子及其基因相互作用网络。基于社区的疫苗本体和布鲁氏菌病本体的发展为布鲁氏菌病数据的集成、交换和计算机辅助自动推理提供了有效的途径。
Brucella is a Gram-negative, facultative intracellular bacterium that causes zoonotic brucellosis in humans and various animals. Out of 10 classified Brucella species, B. melitensis, B. abortus, B. suis, and B. canis are pathogenic to humans. In the past decade, the mechanisms of Brucella pathogenesis and host immunity have been extensively investigated using the cutting edge systems biology and bioinformatics approaches. This article provides a comprehensive review of the applications of Omics (including genomics, transcriptomics, and proteomics) and bioinformatics technologies for the analysis of Brucella pathogenesis, host immune responses, and vaccine targets. Based on more than 30 sequenced Brucella genomes, comparative genomics is able to identify gene variations among Brucella strains that help to explain host specificity and virulence differences among Brucella species. Diverse transcriptomics and proteomics gene expression studies have been conducted to analyze gene expression profiles of wild type Brucella strains and mutants under different laboratory conditions. High throughput Omics analyses of host responses to infections with virulent or attenuated Brucella strains have been focused on responses by mouse and cattle macrophages, bovine trophoblastic cells, mouse and boar splenocytes, and ram buffy coat. Differential serum responses in humans and rams to Brucella infections have been analyzed using high throughput serum antibody screening technology. The Vaxign reverse vaccinology has been used to predict many Brucella vaccine targets. More than 180 Brucella virulence factors and their gene interaction networks have been identified using advanced literature mining methods. The recent development of community-based Vaccine Ontology and Brucellosis Ontology provides an efficient way for Brucella data integration, exchange, and computer-assisted automated reasoning.
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影响因子：
1.3
作者：
Arenas-Gamboa, Angela M.;Ficht, Thomas A.;Rice-Ficht, Allison C.
通讯作者：
Rice-Ficht, Allison C.
影响因子：
3.7
作者：
Chen F;He Y
通讯作者：
He Y
DOI：
10.3181/0904-rm-124
发表时间：
2009-12
期刊：
Experimental biology and medicine (Maywood, N.J.)
影响因子：
--
作者：
Covert J;Mathison AJ;Eskra L;Banai M;Splitter G
通讯作者：
Splitter G
影响因子：
3.1
作者：
Chain, PSG;Comerci, DJ;Garcia, E
通讯作者：
Garcia, E
DOI：
10.1073/pnas.221575398
发表时间：
2002-01-08
影响因子：
11.1
作者：
DelVecchio, VG;Kapatral, V;Overbeek, R
通讯作者：
Overbeek, R