Chilling Stress Triggers VvAgo1-Mediated miRNA-Like RNA Biogenesis in Volvariella volvacea.
Chilling Stress Triggers VvAgo1-Mediated miRNA-Like RNA Biogenesis in Volvariella volvacea.
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DOI：
10.3389/fmicb.2020.523593
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发表时间：
2020
影响因子：
5.2
通讯作者：
Bao D
中科院分区：
文献类型：
--
作者：
Gong M;Wang Y;Zhang J;Zhao Y;Wan J;Shang J;Yang R;Wu Y;Li Y;Tan Q;Bao D
草菇是一种重要的食用菌，低温自溶是其代谢异常的典型现象。基因表达谱和qPCR分析表明，低温胁迫（CS）仅显著且持续地上调草菇中的一种Argonaute，即，VvAgo1.结构和进化分析表明，VvAgo 1属于Ago-like家族，其进化涉及基因复制，随后的基因丢失和纯化选择。其相互作用网络和表达的分析表明，CS触发草菇V23中VvAgo 1介导的miRNA样RNA（milRNA）生物合成，但不触发VH 3（来自V23的具有改善的CS抗性的复合突变株）。小RNA测序和qPCR分析证实CS触发V23中而不是VH 3中的milRNA表达增加。增加的milRNA的预测靶基因在几个途径中富集，例如信号转导和泛素化。热图分析表明，CS改变了V23中与信号转导和泛素化相关的milRNA及其靶基因的表达谱。转录组和蛋白质组数据的组合分析证实，增加的milRNA的大部分靶基因没有翻译成蛋白质。我们的观察表明，CS可能触发VvAgo 1介导的RNAi，以促进草菇的低温自溶。
In Volvariella volvacea, an important species of edible mushroom, cryogenic autolysis is a typical phenomenon that occurs during abnormal metabolism. Analysis of gene expression profiling and qPCR showed that chilling stress (CS) significantly and continuously upregulated only one type of Argonaute in V. volvacea, i.e., VvAgo1. Structural and evolutionary analysis revealed that VvAgo1 belongs to the Ago-like family, and its evolution has involved gene duplication, subsequent gene loss, and purifying selection. Analysis of its interaction network and expression suggested that CS triggers VvAgo1-mediated miRNA-like RNA (milRNA) biogenesis in V. volvacea V23 but not in VH3 (a composite mutant strain from V23 with improved CS resistance). Small RNA sequencing and qPCR analysis confirmed that CS triggered the increased milRNA expression in V23 and not in VH3. The predicted target genes of the increased milRNAs were enriched in several pathways, such as signal transduction and ubiquitination. Heatmap analysis showed that CS altered the expression profile of milRNAs with their target genes related to signal transduction and ubiquitination in V23. Combined analysis of transcriptome and proteome data confirmed that most of the target genes of the increased milRNAs were not translated into proteins. Our observations indicate that CS might trigger VvAgo1-mediated RNAi to facilitate the cryogenic autolysis of V. volvacea.
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影响因子：
6.5
作者：
Anisimova M;Gil M;Dufayard JF;Dessimoz C;Gascuel O
通讯作者：
Gascuel O
杏鲍菇托里菌亚种菌丝体对冷刺激反应的从头测序和转录组分析
DOI：
10.3390/molecules21050560
发表时间：
2016-05-17
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Fu YP;Liang Y;Dai YT;Yang CT;Duan MZ;Zhang Z;Hu SN;Zhang ZW;Li Y
通讯作者：
Li Y
影响因子：
64.5
作者：
Elkayam E;Kuhn CD;Tocilj A;Haase AD;Greene EM;Hannon GJ;Joshua-Tor L
通讯作者：
Joshua-Tor L
影响因子：
2.8
作者：
Hu, Yang;Stenlid, Jan;Olson, Ake
通讯作者：
Olson, Ake
影响因子：
3.3
作者：
Gong, Ming;Li, Zhengpeng;Bao, Dapeng
通讯作者：
Bao, Dapeng