Experimental Actinobacillus pleuropneumoniae challenge in swine: comparison of computed tomographic and radiographic findings during disease.
Experimental Actinobacillus pleuropneumoniae challenge in swine: comparison of computed tomographic and radiographic findings during disease.
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DOI：
10.1186/1746-6148-8-47
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发表时间：
2012-04-30
影响因子：
2.6
通讯作者：
Waldmann KH
中科院分区：
文献类型：
--
作者：
Brauer C;Hennig-Pauka I;Hoeltig D;Buettner FF;Beyerbach M;Gasse H;Gerlach GF;Waldmann KH
在猪中，由胸膜肺炎放线杆菌（App）感染引起的胸膜肺炎等呼吸道疾病几十年来造成了巨大的经济损失。需要进一步研究疾病的发病机制、病原体-宿主相互作用以及新的预防和治疗方法。在大多数研究中，需要大量实验动物来评估疾病不同阶段的肺部变化。为了减少所需的动物数量，同时收集有关活猪肺改变的性质和程度的信息，开发了一种用于量化大体病理结果的计算机断层扫描评分系统。本研究选用5头健康猪作为对照，24头猪感染猪胸膜肺炎病原体App，建立呼吸道疾病模型。通过影像学、临床、血清学、大体病理和组织学检查验证App侵袭过程中的CT表现。在刺激后第7天和第21天记录临床检查、CT和放射成像结果。实验性App刺激后的临床体征可指示急性到慢性疾病。感染猪的肺部CT表现包括磨玻璃影和实变。在第7天和第21天，临床评分与两种成像技术的评分显著相关。第21天，临床评分、CT评分和肺病变评分之间存在显著相关性。22头感染猪中有19头的CT和大体病理检查确定的疾病等级（未感染、轻度感染、中度感染、严重感染）一致。24头猪中有11头猪的x线摄影和大体病理学的疾病分类一致。高分辨率、高对比度、无脏器重叠的CT检查在评估App侵袭后肺病变方面优于x线摄影。新的CT评分系统可以量化生猪的大体病理性肺改变。然而，计算机断层扫描结果并不能说明呼吸道疾病的病因。
In pigs, diseases of the respiratory tract like pleuropneumonia due to Actinobacillus pleuropneumoniae (App) infection have led to high economic losses for decades. Further research on disease pathogenesis, pathogen-host-interactions and new prophylactic and therapeutic approaches are needed. In most studies, a large number of experimental animals are required to assess lung alterations at different stages of the disease. In order to reduce the required number of animals but nevertheless gather information on the nature and extent of lung alterations in living pigs, a computed tomographic scoring system for quantifying gross pathological findings was developed. In this study, five healthy pigs served as control animals while 24 pigs were infected with App, the causative agent of pleuropneumonia in pigs, in an established model for respiratory tract disease. Computed tomographic (CT) findings during the course of App challenge were verified by radiological imaging, clinical, serological, gross pathology and histological examinations. Findings from clinical examinations and both CT and radiological imaging, were recorded on day 7 and day 21 after challenge. Clinical signs after experimental App challenge were indicative of acute to chronic disease. Lung CT findings of infected pigs comprised ground-glass opacities and consolidation. On day 7 and 21 the clinical scores significantly correlated with the scores of both imaging techniques. At day 21, significant correlations were found between clinical scores, CT scores and lung lesion scores. In 19 out of 22 challenged pigs the determined disease grades (not affected, slightly affected, moderately affected, severely affected) from CT and gross pathological examination were in accordance. Disease classification by radiography and gross pathology agreed in 11 out of 24 pigs. High-resolution, high-contrast CT examination with no overlapping of organs is superior to radiography in the assessment of pneumonic lung lesions after App challenge. The new CT scoring system allows for quantification of gross pathological lung alterations in living pigs. However, computed tomographic findings are not informative of the etiology of respiratory disease.
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影响因子：
2.4
作者：
LIGGETT, AD;HARRISON, LR;FARRELL, RL
通讯作者：
FARRELL, RL
影响因子：
2.4
作者：
HANNAN, PCT;BHOGAL, BS;FISH, JP
通讯作者：
FISH, JP
影响因子：
5
作者：
ENGELER, CE;TASHJIAN, JH;WALSH, JW
通讯作者：
WALSH, JW
影响因子：
1.3
作者：
KALENDER, WA;FICHTE, H;SKALEJ, M
通讯作者：
SKALEJ, M
DOI：
10.1111/j.1740-8261.1981.tb01694.x
发表时间：
1981-01-01
期刊：
VETERINARY RADIOLOGY
影响因子：
--
作者：
FARROW, CS
通讯作者：
FARROW, CS