β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress.
β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress.
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DOI：
10.1186/s43141-021-00183-6
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发表时间：
2021-05-29
期刊：
影响因子：
--
通讯作者：
Nishawy E
中科院分区：
文献类型：
--
作者：
Shahzad R;Ewas M;Harlina PW;Khan SU;Zhenyuan P;Nie X;Nishawy E
紫外线-B（UV-B）辐射的增加对许多生物体特别是农作物具有潜在的危害，已成为一个全球性的挑战。水稻是世界范围内种植的一种非常重要的主食，最近已经做了许多努力来改进水稻品种以抵抗UV-B胁迫。研究了外源β-谷甾醇（β-Sito）对水稻生长的促进作用及其对长时间UV-B胁迫的耐受性。在不补充βSito（Nβ）和补充βSito（Sβ）的水稻植株中评价了生理和代谢反应。Nβ和Sβ植株分别在非胁迫（ns）和长时间UV-B胁迫（uvs）条件下生长，并分别称为Nβns、Sβns和Nβuvs、Sβuvs。在非胁迫条件下，特别是在UV-B胁迫条件下，与Nβ植物相比，βSito的应用在改善与生长和发育相关的许多生理参数方面做出了积极贡献，例如Sβ中的芽和根长度、RWC、整株植物生物量、叶绿素色素和光合相关参数（Pn、Gs、Tr、WUEI、Fv/Fm和NPQ）。Sβuvs和Nβuvs植物在氧化胁迫下的耐受性增强，这是由于较低的ROS水平和抗氧化酶（SOD、POD、CAT和APX）活性的大量触发所致。利用GC-TOFMS进行代谢分析，其显示Sβuvs中的几种关键代谢物（包括有机酸、糖、氨基酸等）的积累高于Nβuvs植物，而在胁迫条件下与非胁迫条件下，Nβ植物中的这些代谢物主要减少。这些结果提供了有用的数据，βSito的重要作用，在生长的维持和调节的几种代谢产物与渗透和氧化还原调节在水稻植物的UV-B胁迫耐受性。重要的是，β Sito调节的可塑性可以进一步探索，特别是在其他经济有用的作物植物中与不同的环境胁迫的关系。在线版本包含补充材料，可通过10.1186/s43141-021-00183-6获得。
Elevated ultraviolet-B (UV-B) radiation is potentially deleterious to many organisms specifically crop plants and has become a global challenge. Rice is an exceptionally important staple food which is grown worldwide, and many efforts have been done recently to improve rice varieties against UV-B stress. This current study aims to investigate the effects of exogenous application of β-sitosterol (βSito) on growth improvement and tolerance level of rice plants against prolonged UV-B stress. The physiological and metabolic responses were evaluated in rice plants not supplemented with βSito (Nβ) and those supplemented with βSito (Sβ). The Nβ and Sβ plants were grown under non-stress (ns) and under prolonged UV-B stress (uvs) conditions and termed as Nβns, Sβns and Nβuvs, Sβuvs, respectively. The application of βSito contributes positively under non-stress and specifically to UV-B stress in terms of improving numerous physiological parameters associated with growth and development such as shoot and root length, RWC, whole plant biomass, chlorophyll pigments, and photosynthetic-related parameters (Pn, Gs, Tr, WUEi, Fv/Fm, and NPQ) in Sβ compared with Nβ plants. Moreover, enhanced oxidative stress tolerance of Sβuvs vs. Nβuvs plants under stress was attributed to low levels of ROS and substantial trigger in activities of antioxidant enzymes (SOD, POD, CAT, and APX). Metabolic analysis was performed using GC-TOFMS, which revealed higher accumulation of several key metabolites including organic acids, sugars, amino acids, and others in Sβuvs vs. Nβuvs plants, which were mainly reduced in Nβ plants under stress vs. non-stress conditions. These results provide useful data regarding the important role of βSito on growth maintenance and modulation of several metabolites associated with osmotic and redox adjustments during UV-B stress tolerance in rice plants. Importantly, βSito-regulated plasticity could further be explored specifically in relation to different environmental stresses in other economically useful crop plants. The online version contains supplementary material available at 10.1186/s43141-021-00183-6.
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