Metabolic and physiological responses to progressive drought stress in bread wheat.
Metabolic and physiological responses to progressive drought stress in bread wheat.
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面包小麦对渐进性干旱胁迫的代谢和生理响应。
DOI：
10.1038/s41598-020-74303-6
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发表时间：
2020-10-14
影响因子：
4.6
通讯作者：
Tsujimoto H
中科院分区：
文献类型：
--
作者：
Itam M;Mega R;Tadano S;Abdelrahman M;Matsunaga S;Yamasaki Y;Akashi K;Tsujimoto H
小麦（Tritium aestivum）易受未来气候变化的影响，因为它主要在干旱地区的雨水灌溉条件下种植。因此，深入了解干旱对小麦代谢的影响对培育耐旱小麦品种至关重要。在这里，我们暴露小麦'Norin 61'植物在开花期进行性干旱胁迫[0（干旱前），2，4，6，8，和10天后扣水]，以研究生理和代谢的反应。关键脱落酸响应基因的转录分析表明，脱落酸信号在小麦适应水分亏缺中发挥了重要作用。干旱胁迫下，冠层温度（CT）升高，碳同位素组成值高于对照。CT下降与土壤水势（SWP）密切相关。此外，-517 kPa的SWP被确定为增加CT和诱导活性氧的临界点。代谢组学分析确定了四个潜在的干旱响应生物标志物，通过嘌呤和嘧啶代谢的氮循环的增强，干旱诱导的衰老的基础上1-氨基环丙烷-1-羧酸和天冬酰胺的积累，并通过血清素积累在严重干旱胁迫下的抗衰老反应。我们的研究结果提供了深入了解分子，生理和代谢变化参与干旱反应，这是有用的小麦育种计划，以开发耐旱小麦品种。
Wheat (Tritium aestivum) is vulnerable to future climate change because it is predominantly grown under rain-fed conditions in drought-prone areas. Thus, in-depth understanding of drought effect on wheat metabolism is essential for developing drought-tolerant wheat varieties. Here, we exposed wheat ‘Norin 61’ plants to progressive drought stress [0 (before drought), 2, 4, 6, 8, and 10 days after withholding water] during the flowering stage to investigate physiological and metabolomic responses. Transcriptional analyses of key abscisic acid-responsive genes indicated that abscisic acid signalling played a major role in the adaptation of wheat to water deficit. Carbon isotope composition had a higher value than the control while canopy temperature (CT) increased under drought stress. The CT depression was tightly correlated with soil water potential (SWP). Additionally, SWP at − 517 kPa was identified as the critical point for increasing CT and inducing reactive oxygen species. Metabolome analysis identified four potential drought-responsive biomarkers, the enhancement of nitrogen recycling through purine and pyrimidine metabolism, drought-induced senescence based on 1-aminocyclopropane-1-carboxylic acid and Asn accumulation, and an anti-senescence response through serotonin accumulation under severe drought stress. Our findings provide in-depth insight into molecular, physiological and metabolite changes involved in drought response which are useful for wheat breeding programs to develop drought-tolerant wheat varieties.
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DOI：
10.1007/s00122-018-3102-x
发表时间：
2018-08
期刊：
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
影响因子：
--
作者：
Gorafi YSA;Kim JS;Elbashir AAE;Tsujimoto H
通讯作者：
Tsujimoto H
影响因子：
56.9
作者：
BOYER, JS
通讯作者：
BOYER, JS
通过增加拟南芥和尼古丁塔巴库姆的磷酸糖基磷酸合成酶活性来增加生物量的积累。
影响因子：
13.8
作者：
Koslowsky, Silke;Riegler, Heike;Bergmueller, Eveline;Zrenner, Rita
通讯作者：
Zrenner, Rita
影响因子：
6.9
作者：
Dubouzet, Joseph G.;Ishihara, Atsushi;Wakasa, Kyo
通讯作者：
Wakasa, Kyo
影响因子：
3.7
作者：
Mason, R. Esten;Singh, Ravi P.
通讯作者：
Singh, Ravi P.