Low frequency stimulation decreases seizure activity in a mutation model of epilepsy.
Low frequency stimulation decreases seizure activity in a mutation model of epilepsy.
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DOI：
10.1111/j.1528-1167.2010.02679.x
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发表时间：
2010-09
期刊：
影响因子：
5.6
通讯作者：
Durand DM
中科院分区：
文献类型：
--
作者：
Kile KB;Tian N;Durand DM
目的 研究因 Scn2a 钠通道基因功能获得性突变而表现出自发性癫痫发作的 Q54 小鼠的脑电活动，并评估低频深部脑刺激 (DBS) 对降低癫痫发作频率的功效。将 EEG、EMG 和海马深部电极植入表达癫痫表型的 Q54 小鼠 (n = 6)。长期六通道录音（宽带，0.1–300 Hz）每天 24 小时存储超过 12 天。低频刺激 (LFS)（3Hz，方波，双相，100μs，400μA）以交替的五分钟周期（开或关）每天 24 小时应用于腹侧海马连合（VHC），持续四天。当应用于癫痫 Q54 小鼠 (n = 6) 的 VHC 时，LFS (3Hz) 导致癫痫发作频率和持续时间显着减少（21% 和 35%，p<0.05）。癫痫发作频率不受刺激状态（“开”与“关”）的直接影响。在 Q54 模型中，以 3Hz 频率应用 LFS 显着降低了癫痫发作频率和持续时间。此外，LFS 不会立即减少癫痫发作频率和持续时间，而是具有延迟和持久的效果，支持复杂的间接作用机制。
To investigate brain electrical activity in Q54 mice that display spontaneous seizures because of a gain-of-function mutation of the Scn2a sodium channel gene, and to evaluate the efficacy of low frequency deep brain stimulation (DBS) for seizure frequency reduction. EEG, EMG, and hippocampal deep electrodes were implanted into Q54 mice expressing an epileptic phenotype (n = 6). Chronic six channel recordings (wideband, 0.1–300 Hz) were stored 24 hours a day for more than 12 days. Low Frequency stimulation (LFS) (3Hz, square wave, biphasic, 100μs, 400μA) was applied to the ventral hippocampal commisure (VHC) in alternating five minute cycles (on or off) 24 hours a day for a period of four days. LFS (3Hz) resulted in a significant reduction in seizure frequency and duration (21% and 35%, p<0.05), when applied to the VHC of epileptic Q54 mice (n = 6). Seizure frequency was not directly affected by stimulation state (“on” versus “off”). LFS applied at a frequency of 3Hz significantly reduced seizure frequency and duration in the Q54 model. Furthermore, the reduction of seizure frequency and duration by LFS was not immediate but had a delayed and lasting effect, supporting complex, indirect mechanisms of action.
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影响因子：
5.3
作者：
GODDARD, GV;MCINTYRE, DC;LEECH, CK
通讯作者：
LEECH, CK
影响因子：
2.5
作者：
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影响因子：
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作者：
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影响因子：
5.3
作者：
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通讯作者：
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