Comparative microarray profile of the hepatopancreas in the response of "Huanghai No. 2" Fenneropenaeus chinensis to white spot syndrome virus.
Comparative microarray profile of the hepatopancreas in the response of "Huanghai No. 2" Fenneropenaeus chinensis to white spot syndrome virus.
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中国明对虾“黄海2号”对白斑综合症病毒反应的肝胰腺微阵列图谱比较。
DOI：
10.1016/j.fsi.2016.07.031
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Li Xupeng
中科院分区：
文献类型：
--
作者：
Shi Xiaoli;Kong Jie;Meng Xianhong;Luan Sheng;Luo Kun;Cao Baoxiang;Liu Ning;Lu Xia;Deng Kangyu;Cao Jiawang;Zhang Yingxue;Zhang Hengheng;Li Xupeng
对虾白斑综合征病毒(WSSV)侵染所有对虾品种，对对虾养殖危害最大。为了更好地了解黄海2号中国对虾对WSSV感染的分子应答机制，采用了基因芯片技术。微阵列基因表达谱对59,137个非基因在活虾和死虾早期、高峰期和晚期的差异表达基因(DEG)进行了鉴定。在活体对虾中，早期、高峰期和晚期分别为1307、1479和1539℃。同时，在濒死对虾中获得了1536、2181和1591个温度。已知的20个注释基因在活体对虾后期唯一表达，包括黏附调节分子1、精氨酸激酶、BUD31同源物和QM。与WSSV敏感对虾相比，已知的75个注释基因在WSSV抗性对虾中唯一表达，包括精氨酸激酶、BUD31同源物、可凝结蛋白2、半胱氨酸蛋白酶2、组织蛋白酶C、钙蛋白、HMGBb、组蛋白3和硒蛋白M。为了进一步证实差异表达基因的表达，我们采用实时荧光定量RT-PCR技术检测了6个随机选择的基因。这些数据将为理解对虾对WSSV的免疫应答机制提供有价值的信息。
White spot syndrome virus (WSSV) infects all shrimp species and is the greatest detriment to shrimp culture. To better understand the mechanism of molecular responses to WSSV infection in “Huanghai No. 2”Fenneropenaeus chinensis, a microarray technique was used. Microarray gene expression profiling of 59,137 unigenes identified Differentially Expressed Genes (DEGs) both in live and moribund shrimp at early, peak and late phases. In live shrimp, 1307, 1479 and 1539 DEGs were obtained in the early, peak and late phase, respectively. Meanwhile, 1536, 2181 and 1591 DEGs were obtained in moribund shrimp. Twenty known annotation genes are uniquely expressed in the late phase of live shrimp, including adhesion regulating molecule 1, arginine kinase, BUD31 homolog, and QM. Compared to WSSV-susceptible shrimp, 75 known annotation genes are uniquely expressed in WSSV-resistant shrimp, including arginine kinase, BUD31 homolog, clottable protein 2, caspase 2, cathepsin C, calnexin, HMGBb, Histone 3, and selenoprotein M. The gene expression patterns of the infected shrimp were altered by WSSV infection. To further confirm the expression of differentially expressed genes, real-time RT-PCR was performed to test six randomly selected genes. The data will provide valuable information to understand the immune mechanism of shrimp's response to WSSV.
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影响因子：
1.2
作者：
Thisse, C;Degrave, A;Lescure, A
通讯作者：
Lescure, A
影响因子：
--
作者：
Jianyang Xu;Suijie Wu;Xiaobo Zhang
通讯作者：
Jianyang Xu;Suijie Wu;Xiaobo Zhang
影响因子：
4.2
作者：
Rao R;Bing Zhu Y;Alinejad T;Tiruvayipati S;Lin Thong K;Wang J;Bhassu S
通讯作者：
Bhassu S
DOI：
--
发表时间：
2012-10
期刊：
--
影响因子：
--
作者：
I. Müller
通讯作者：
I. Müller
影响因子：
4.7
作者：
C. Yao;Changgong Wu;J. Xiang;Bo Dong
通讯作者：
C. Yao;Changgong Wu;J. Xiang;Bo Dong