Serum Immune Profiling for Early Detection of Cervical Disease.
Serum Immune Profiling for Early Detection of Cervical Disease.
复制标题
DOI：
10.7150/thno.21098
复制
发表时间：
2017
期刊：
影响因子：
12.4
通讯作者：
Anderson KS
中科院分区：
文献类型：
--
作者：
Ewaisha R;Panicker G;Maranian P;Unger ER;Anderson KS
背景资料：国际癌症研究机构最近（2012年）的全球估计表明，全球每年约有528，000例新病例和270，000例死亡归因于宫颈癌。这种疾病是可以预防的HPV疫苗接种和早期发现和治疗浸润前宫颈上皮内瘤变，CIN。针对HPV蛋白的抗体（Abs）作为早期检测的潜在生物标志物正在研究中。研究方法：为了检测循环HPV特异性IgG抗体，我们开发了可编程蛋白阵列（NAPPA），显示两种低风险HPV类型（HPV 6和11）和十种致癌高危HPV类型（HPV 16，18，31，33，35，39，45，51，52和58）的蛋白质组。用来自患有CIN 0/I（n=78）、CIN II/III（n=84）或浸润性宫颈癌（ICC，n=83）的女性的血清探测阵列。结果如下：CIN 0/I组（23.7%）对任何早期（E）HPV蛋白的抗体检出率低于CINII/III组（39.0%）和ICC组（46.1%，p<0.04）。在E Ab中，抗E7 Ab最常检出（分别为6.6%、19.5%和30.3%）。最少检出的Ab为CIN 0/I中的E1和E2-Ab（1.3%），CIN II/III（1.2%）和ICC（7.9%）中的E1-Ab。在CIN 0/I、CIN II/III和ICC中，HPV 16特异性抗体与宫颈中检测到的HPV 16 DNA的相关性分别为0%、21.2%和45.5%。大量（29 - 73%）E4、E7、L1和L2抗体在HPV类型之间具有交叉反应性。结论：HPV蛋白芯片提供了一个有价值的高通量工具，用于测量宫颈疾病中HPV血清学反应的广度、特异性和异质性。
Background: The most recent (2012) worldwide estimates from International Agency for Research on Cancer indicate that approximately 528,000 new cases and 270,000 deaths per year are attributed to cervical cancer worldwide. The disease is preventable with HPV vaccination and with early detection and treatment of pre-invasive cervical intraepithelial neoplasia, CIN. Antibodies (Abs) to HPV proteins are under investigation as potential biomarkers for early detection. Methods: To detect circulating HPV-specific IgG Abs, we developed programmable protein arrays (NAPPA) that display the proteomes of two low-risk HPV types (HPV6 and 11) and ten oncogenic high-risk HPV types (HPV16, 18, 31, 33, 35, 39, 45, 51, 52 and 58). Arrays were probed with sera from women with CIN 0/I (n=78), CIN II/III (n=84), or invasive cervical cancer (ICC, n=83). Results: Abs to any early (E) HPV protein were detected less frequently in women with CIN 0/I (23.7%) than women with CIN II/III (39.0%) and ICC (46.1%, p<0.04). Of the E Abs, anti-E7 Abs were the most frequently detected (6.6%, 19.5%, and 30.3%, respectively). The least frequently detected Abs were E1 and E2-Abs in CIN 0/I (1.3%) and E1-Abs in CIN II/III (1.2%) and ICC (7.9%). HPV16-specific Abs correlated with HPV16 DNA detected in the cervix in 0% of CIN 0/I, 21.2% of CIN II/III, and 45.5% of ICC. A significant number (29 - 73%) of E4, E7, L1, and L2 Abs had cross-reactivity between HPV types. Conclusion: HPV protein arrays provide a valuable high-throughput tool for measuring the breadth, specificity, and heterogeneity of the serologic response to HPV in cervical disease.
登录
查看更多内容
DOI：
10.1002/prca.201500096
发表时间：
2016-07
期刊：
Proteomics. Clinical applications
影响因子：
--
作者：
Katchman BA;Barderas R;Alam R;Chowell D;Field MS;Esserman LJ;Wallstrom G;LaBaer J;Cramer DW;Hollingsworth MA;Anderson KS
通讯作者：
Anderson KS
影响因子：
3.4
作者：
Ewaisha R;Meshay I;Resnik J;Katchman BA;Anderson KS
通讯作者：
Anderson KS
影响因子：
4.7
作者：
Kang, Woo Dae;Choi, Ho Sun;Kim, Seok Mo
通讯作者：
Kim, Seok Mo
影响因子：
45.3
作者：
Kreimer, Aimee R.;Johansson, Mattias;Brennan, Paul
通讯作者：
Brennan, Paul
影响因子：
--
作者：
Achour, M.;Zeghal, D.;Oueslati, R.
通讯作者：
Oueslati, R.