An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors.

An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors.
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XA21 相关激酶 (OsSERK2) 调节 X​​A21 和 XA3 免疫受体介导的免疫

DOI:
10.1093/mp/ssu003
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发表时间:
2014-05
期刊:
影响因子:
27.5
通讯作者:
Ronald PC
Ronald PC
中科院分区:
生物学1区
文献类型:
--
作者:
Chen X;Zuo S;Schwessinger B;Chern M;Canlas PE;Ruan D;Zhou X;Wang J;Daudi A;Petzold CJ;Heazlewood JL;Ronald PC

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水稻Xa21免疫受体激酶和结构上相关的Xa3受体对水稻白叶枯病菌具有免疫力。白叶枯病的病原菌--稻瘟病菌。本文报道了水稻体细胞胚胎发生受体蛋白2(OsSERK2)的分离,并证明了OsSERK2对Xa21和Xa3以及水稻免疫受体Fls2(OsFls2)介导的免疫具有正向调节作用。对OsSerk2沉默的水稻植株表现出形态变化和对激素油菜素内酯的敏感性降低。在酵母双杂交系统中,OsSERK2与每个免疫受体的胞内区以依赖的方式相互作用。OsSERK2在体外与Xa21发生双向反式磷酸化反应,在体内与Xa21形成构成性复合体。综上所述,这些结果表明OsSERK2在三个水稻免疫受体的功能中起着重要的作用,并表明与水稻免疫受体的直接相互作用对它们的功能至关重要。
The rice XA21 immune receptor kinase and the structurally related XA3 receptor, confer immunity to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight. Here we report the isolation of OsSERK2 (rice somatic embryogenesis receptor kinase 2) and demonstrate that OsSERK2 positively regulates immunity mediated by XA21 and XA3 as well as the rice immune receptor FLS2 (OsFLS2). Rice plants silenced for OsSerk2 display altered morphology and reduced sensitivity to the hormone brassinolide. OsSERK2 interacts with the intracellular domains of each immune receptor in the yeast-two-hybrid system in a kinase activity dependent manner. OsSERK2 undergoes bidirectional trans-phosphorylation with XA21 in vitro and forms a constitutive complex with XA21 in vivo. Taken together, these results demonstrate an essential role for OsSERK2 in the function of three rice immune receptors and suggest that direct interaction with the rice immune receptors is critical for their function.
DOI: 10.1105/tpc.105.036814
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