Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time.
Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time.
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与激发激光器相匹配的短波红外多胸荧光团可实时实时非侵入性，多色在体内成像。
DOI：
10.1038/s41557-020-00554-5
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发表时间：
2020-12
期刊：
影响因子：
21.8
通讯作者：
Sletten EM
中科院分区：
文献类型：
--
作者：
Cosco ED;Spearman AL;Ramakrishnan S;Lingg JGP;Saccomano M;Pengshung M;Arús BA;Wong KCY;Glasl S;Ntziachristos V;Warmer M;McLaughlin RR;Bruns OT;Sletten EM
高分辨率、多路复用实验是细胞成像中的主要内容。然而，由于在可见光（维斯，350-700 nm）和近红外（NIR，700-1000 nm）波长下组织中的显著散射和自发荧光，动物中的类似实验是具有挑战性的。在这里，我们通过短波红外（SWIR，1000-2000 nm）区域的光学造影剂的设计和成像技术的互补进展，实现了实时，非侵入性的动物MRI成像实验。我们开发了可调谐的，短波红外发射黄酸聚甲川染料，并建立了这类明亮的短波红外造影剂的结构-物理性质的关系。同时，我们设计了一个成像系统，可变近红外/短波红外激发和单通道检测，促进视频速率的短波红外成像的光学引导手术和成像的清醒和移动的小鼠与多路检测。与980 nm和1064 nm激光相匹配的优化染料与临床批准的吲哚菁绿色相结合，实现了具有高时间和空间分辨率的实时三色成像。
High resolution, multiplexed experiments are a staple in cellular imaging. Analogous experiments in animals are challenging, however, due to significant scattering and autofluorescence in tissue at visible (VIS, 350–700 nm) and near-infrared (NIR, 700–1000 nm) wavelengths. Here, we enable real-time, non-invasive multicolor imaging experiments in animals through the design of optical contrast agents for the shortwave infrared (SWIR, 1000–2000 nm) region and complementary advances in imaging technologies. We developed tunable, SWIR-emissive flavylium polymethine dyes and established structure-photophysical property relationships for this class of bright SWIR contrast agents. In parallel, we designed an imaging system with variable NIR/SWIR excitation and single-channel detection, facilitating video-rate multicolor SWIR imaging for optically guided surgery and imaging of awake and moving mice with multiplexed detection. Optimized dyes matched to 980 nm and 1064 nm lasers, combined with the clinically approved indocyanine green, enabled real-time, three-color imaging with high temporal and spatial resolutions.
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影响因子：
5.5
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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