Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy

Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy
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通过体内受激拉曼散射显微镜对非洲爪蟾蝌蚪髓鞘形成进行无标记实时成像

DOI:
10.1117/1.jbo.19.8.086005
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发表时间:
2014-08
影响因子:
3.5
通讯作者:
Hu, Bing
Hu, Bing
中科院分区:
医学3区
文献类型:
--
作者:
Zhang, Delong;Slipchenko, Mikhail N.;Cheng, Ji-Xin;Hu, Bing

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抽象。髓鞘作为轴突在神经系统的功能中起重要作用,并且髓鞘降解是多发性硬化和脊髓损伤的标志性病理学。电子显微镜、荧光显微镜和磁共振成像是用于髓鞘可视化的三种主要技术。然而,活生物体中髓鞘的显微镜观察仍然是一个挑战。使用一种新开发的受激拉曼散射显微镜的方法,我们报告的非侵入性,无标记,实时在体内成像的髓鞘形成由一个单一的雪旺氏细胞,成熟的一个节点的朗维尔,和髓鞘降解的透明体的非洲爪蟾蝌蚪。
Abstract. The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time in vivo imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the Xenopus laevis tadpole.
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