Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis.
Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis.
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DOI：
10.1083/jcb.98.6.1926
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发表时间：
1984-06
期刊：
影响因子：
--
通讯作者：
Fambrough DM
中科院分区：
文献类型：
--
作者：
Chiquet M;Fambrough DM
细胞外基质成分可能参与肌肉与非肌肉细胞在形态发生和成人骨骼肌中的相互作用。为了鉴定相关分子，我们产生了与肌内膜反应的单克隆抗体，即，单个肌纤维表面的细胞外基质。本文所述的抗体M1特异性标记鸡背阔肌前肌的肌内膜（但既不标记肌束膜，也不标记肌外膜，血管和神经束膜除外）。肌内膜标记仅限于近端和远端部分的肌纤维附近的插入点的肌腱，但没有从内侧区域的肌肉。M1抗体强烈标记肌腱连接处和腱束。在鸡胚中，“肌腱抗原”（我们暂时称之为M1抗体识别的表位）首先出现在椎骨和四肢软骨成分的软骨膜中，从那里它逐渐延伸到前肌块。第6天左右，肌腱原基被清晰标记。鸡胚中M1抗体标记的其他结构是发育中的平滑肌组织，尤其是主动脉、肌胃和肺芽。一般而言，用M1抗体标记的组织似乎是积聚纤连蛋白的组织的子集。在细胞培养物中，M1抗体与活成纤维细胞和肌原细胞的基质和细胞表面上的模糊纤维状物质结合，这证实了抗原位点的细胞外位置。肌腱抗原在肢体形态发生期间的出现及其在成人肌肉和肌腱中的分布与它可能参与将肌纤维附着到肌腱束的想法是一致的。其生物化学表征描述于随附的论文（Chiquet，M.，和D. Fambrough，1984，J. Cell Biol.98：1937-1946）。
Extracellular matrix components are likely to be involved in the interaction of muscle with nonmuscle cells during morphogenesis and in adult skeletal muscle. With the aim of identifying relevant molecules, we generated monoclonal antibodies that react with the endomysium, i.e., the extracellular matrix on the surface of single muscle fibers. Antibody M1, which is described here, specifically labeled the endomysium of chick anterior latissimus dorsi muscle (but neither the perimysium nor, with the exception of blood vessels and perineurium, the epimysium ). Endomysium labeling was restricted to proximal and distal portions of muscle fibers near their insertion points to tendon, but absent from medial regions of the muscle. Myotendinous junctions and tendon fascicles were intensely labeled by M1 antibody. In chick embryos, " myotendinous antigen" (as we tentatively call the epitope recognized by M1 antibody) appeared first in the perichondrium of vertebrae and limb cartilage elements, from where it gradually extended to the premuscle masses. Around day 6, tendon primordia were clearly labeled. The other structures labeled by M1 antibody in chick embryos were developing smooth muscle tissues, especially aorta, gizzard, and lung buds. In general, tissues labeled with M1 antibody appeared to be a subset of the ones accumulating fibronectin. In cell cultures, M1 antibody binds to fuzzy, fibrillar material on the substrate and cell surfaces of living fibroblast and myogenic cells, which confirms an extracellular location of the antigenic site. The appearance of myotendinous antigen during limb morphogenesis and its distribution in adult muscle and tendon are compatible with the idea that it might be involved in attaching muscle fibers to tendon fascicles. Its biochemical characterization is described in the accompanying paper ( Chiquet , M., and D. Fambrough , 1984, J. Cell Biol. 98:1937-1946).
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影响因子：
4.1
作者：
GREENWOOD, FC;HUNTER, WM
通讯作者：
HUNTER, WM
影响因子：
7.8
作者：
CHIQUET, M;FAMBROUGH, DM
通讯作者：
FAMBROUGH, DM
DOI：
10.1083/jcb.95.2.369
发表时间：
1982-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hynes RO;Yamada KM
通讯作者：
Yamada KM
影响因子：
7.8
作者：
GARDNER, JM;FAMBROUGH, DM
通讯作者：
FAMBROUGH, DM
影响因子：
15.3
作者：
LINDER, E;VAHERI, A;WARTIOVAARA, J
通讯作者：
WARTIOVAARA, J