Mitogenomic analysis of the genus Panthera
Mitogenomic analysis of the genus Panthera
复制标题
豹属的线粒体基因组分析
DOI：
10.1007/s11427-011-4219-1
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
采用聚合酶链反应方法测定了东北虎、豹和虎豹的线粒体DNA全基因组序列。结果表明，这3个种的线粒体DNA全序列长度分别为16990、16964和16773 bp。三个线粒体DNA基因组中的每一个都包括13个蛋白质编码基因、22个tRNA、2个rRNA、1个O（L）R和1个控制区。基因组的结构与猫卡图斯，Acinonyx jubatus和Neofelis nebulosa的基因组高度相似。根据线粒体基因组全序列和两个线粒体序列数据集，采用最大简约法（MP）、最大似然法（ML）和贝叶斯分析法，对豹属的亲缘关系进行了推断。结果表明，东北虎类由狮子豹、虎豹、豹、虎豹和东北虎组成。nebulosa，作为最基部的成员。Panthera属内的单系发生为N. nebulosa（P. tigris（P. onca（P. pardus，（P. leo，P. uncia））））。基于ML分支长度和四个良好建立的校准点估计了Panthera属的发散时间。结果表明，大约在11.3MYA，Panthera属从其他猫科动物中分离出来，然后进化成该属的几个物种。详细地说，N. nebulosa约8.66 MYA，P. tigris约6.55 MYA，P. uncia约4.63 MYA，P. pardus约4.35 MYA。所有这些估计的时间都比从化石记录中估计的时间要早。作为中亚特有种，以青藏高原及其周边高原为核心生境，P.uncia的分化事件、演化过程、物种形成和分布格局与晚新生代青藏高原8、3.6、2.5和1.7百万年前的地质构造事件和强烈的气候变化密切相关。
The complete sequences of the mitochondrial DNA genomes of Panthera tigris, Panthera pardus, and Panthera uncia were determined using the polymerase chain reaction method. The lengths of the complete mitochondrial DNA sequences of the three species were 16990, 16964, and 16773 bp, respectively. Each of the three mitochondrial DNA genomes included 13 protein-coding genes, 22 tRNA, two rRNA, one O(L)R, and one control region. The structures of the genomes were highly similar to those of Felis catus, Acinonyx jubatus, and Neofelis nebulosa. The phylogenies of the genus Panthera were inferred from two combined mitochondrial sequence data sets and the complete mitochondrial genome sequences, by MP (maximum parsimony), ML (maximum likelihood), and Bayesian analysis. The results showed that Panthera was composed of Panthera leo, P. uncia, P. pardus, Panthera onca, P. tigris, and N. nebulosa, which was included as the most basal member. The phylogeny within Panthera genus was N. nebulosa (P. tigris (P. onca (P. pardus, (P. leo, P. uncia)))). The divergence times for Panthera genus were estimated based on the ML branch lengths and four well-established calibration points. The results showed that at about 11.3 MYA, the Panthera genus separated from other felid species and then evolved into the several species of the genus. In detail, N. nebulosa was estimated to be founded about 8.66 MYA, P. tigris about 6.55 MYA, P. uncia about 4.63 MYA, and P. pardus about 4.35 MYA. All these estimated times were older than those estimated from the fossil records. The divergence event, evolutionary process, speciation, and distribution pattern of P. uncia, a species endemic to the central Asia with core habitats on the Qinghai-Tibetan Plateau and surrounding highlands, mostly correlated with the geological tectonic events and intensive climate shifts that happened at 8, 3.6, 2.5, and 1.7 MYA on the plateau during the late Cenozoic period.
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影响因子：
4.1
作者：
Li Yu;Qing-wei Li;Oliver A. Ryder;Ya-ping Zhang;Ya-ping Zhang
通讯作者：
Li Yu;Qing-wei Li;Oliver A. Ryder;Ya-ping Zhang;Ya-ping Zhang
DOI：
10.1159/000131806
发表时间：
1982
期刊：
Cytogenetics and cell genetics
影响因子：
--
作者：
D. Wurster‐Hill;W. Centerwall
通讯作者：
D. Wurster‐Hill;W. Centerwall
影响因子：
4.9
作者：
Demin Han;K. Zhou
通讯作者：
Demin Han;K. Zhou
影响因子：
1.9
作者：
L. Werdelin
通讯作者：
L. Werdelin
DOI：
10.1360/04yc0117
发表时间：
2004-12-01
期刊：
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
影响因子：
--
作者：
Sun, Y;Ma, F;Li, QW
通讯作者：
Li, QW