Crystallographic characterization of N-oxide tripod amphiphiles.
Crystallographic characterization of N-oxide tripod amphiphiles.
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DOI：
10.1021/ja9085148
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发表时间：
2010-02-17
影响因子：
15
通讯作者：
Gellman, Samuel H.
中科院分区：
文献类型：
--
作者：
Chae, Pil Seok;Guzei, Ilia A.;Gellman, Samuel H.
三脚架两亲体是为了促进固有膜蛋白在水溶液中的溶解和稳定而设计的；促进结晶是一个长期目标。膜蛋白因其重要的生物学作用而受到广泛关注，但由于膜蛋白在水中的溶解度低，因此很难对其进行研究。通常用于实现溶解度的非离子洗涤剂可能会产生导致蛋白质变性的意想不到的效果。三脚架两亲体与传统洗涤剂的不同之处在于亲脂部分包含一个分支点，以前的工作表明，这种不寻常的两亲结构相对于传统洗涤剂结构是有利的。本文报道了几种含有n -氧化物亲水基团的三脚架两亲体的晶体结构。数据表明，通过改变向非极性表面投射的疏水附属物以及相对于该表面的总体方向，三脚架可以适应非极性表面。虽然不可能从晶体学数据中得出关于溶液中两亲关系的确切结论，但在这里报道的包装模式中观察到的趋势建议在未来的研究中实施设计策略。
Tripod amphiphiles are designed to promote the solubilization and stabilization of intrinsic membrane proteins in aqueous solution; facilitation of crystallization is a long-range goal. Membrane proteins are subjects of extensive interest because of their critical biological roles, but proteins of this type can be difficult to study because of their low solubility in water. The nonionic detergents that are typically used to achieve solubility can have the unintended effect of causing protein denaturation. Tripod amphiphiles differ from conventional detergents in that the lipophilic segment contains a branchpoint, and previous work has shown that this unusual amphiphilic architecture can be advantageous relative to traditional detergent structures. Here we report the crystal structures of several tripod amphiphiles that contain an N-oxide hydrophilic group. The data suggest that tripods can adapt themselves to a nonpolar surface by altering the hydrophobic appendage that projects toward that surface and their overall orientation relative to that surface. Although it is not possible to draw firm conclusions regarding amphiphile association in solution from crystallographic data, trends observed among the packing patterns reported here suggest design strategies to be implemented in future studies.
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影响因子：
2.9
作者：
HJELMELAND, LM;NEBERT, DW;OSBORNE, JC
通讯作者：
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影响因子：
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Belrhali, H;Nollert, P;Pebay-Peyroula, E
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通讯作者：
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作者：
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通讯作者：
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作者：
CHATTOPADHYAY, A;LONDON, E
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