Puerarin attenuates glutamate-induced neurofilament axonal transport impairment.
Puerarin attenuates glutamate-induced neurofilament axonal transport impairment.
复制标题
葛根素可减轻谷氨酸诱导的神经丝轴突运输损伤。
DOI：
10.1016/j.jep.2010.08.005
复制
发表时间：
2010-10
影响因子：
5.4
通讯作者：
中科院分区：
文献类型：
--
作者：
研究目的葛根素 (Pur) 是传统中药中最具功能性的葛根提取物的主要成分，已有数百年历史。由于假设 Pur 可以保护大脑免受谷氨酸 (Glu) 神经毒性，因此我们在本研究中研究了 Pur 对原代海马神经元中 Glu 诱导的轴突运输损伤的影响。 材料和方法 从 2 日龄 Sprague-Dawley 大鼠中制备原代海马培养物。测量细胞内钙浓度 [Ca2+]i、神经丝 (NF) 磷酸化和 Cdk5 的蛋白激酶活性。采用延时成像技术，用瞬时转染的连接至 NF-M N 端的荧光蛋白 (EGFP-NFM) 来捕获培养神经元中 NF 轴突的转运。 结果结果表明，Pur 显着降低了 Glu 诱导的 [Ca2+]iin 剂量依赖性升高，并在蛋白水平上拮抗 Glu 诱发的 NF 磷酸化增加。与对照神经元相比，Glu 处理下的神经元表现出细胞体内不动的 NF 簇的积累，并且 NF 的轴突运输率降低了 72.8%。有趣的是，Pur 将轴突运输减慢的速度逆转了 35.6%。 Pur 还显着减弱 Glu 诱发的 Cdk5 激活。结论 Pur 可能通过抑制 [Ca2+] 增加并阻止 Cdk5 激活，从而在保护大鼠原代海马神经元免受 Glu 诱导的 NF 轴突转运损伤中发挥作用。
AIM OF THE STUDYPuerarin (Pur) is a primary component of the most functional extracts of Pueraria lobata used in traditional Chinese medicine for centuries. Since it has been postulated that Pur protects the brain against glutamate (Glu) neurotoxicity, we investigated the effects of Pur on Glu-induced axonal transport impairment in primary hippocampal neurons in this study.MATERIALS AND METHODSPrimary hippocampal cultures were prepared from 2-day-old Sprague–Dawley rats. Intracellular calcium concentration [Ca2+]i, neurofilament (NF) phosphorylation and protein kinase activity for Cdk5 were measured. Time-lapse imaging technology was used to capture the NF axonal transport in the cultured neurons with transiently transfected fluorescence protein linked to the N-terminus of NF-M (EGFP-NFM).RESULTSThe results showed that Pur significantly diminished the Glu-induced elevation of [Ca2+]iin dose-dependent manner and antagonized the Glu-evoked increases in NF phosphorylation at protein levels. The neurons under the Glu treatment displayed the accumulation of immobile NF clusters in the cell body and the reduced rates of axonal transport of NFs by 72.8% compared to the control neurons. Intriguingly, Pur reversed the slowed rate of the axonal transport by 35.6%. Pur also remarkably attenuated Glu-evoked activation of Cdk5.CONCLUSIONSPur may play a role in protecting against Glu-induced NF axonal transport impairment in rat primary hippocampal neurons by inhibiting the increased [Ca2+]iand by impeding the activation of Cdk5.
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DOI：
10.1083/jcb.200303138
发表时间：
2003-05-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ackerley S;Thornhill P;Grierson AJ;Brownlees J;Anderton BH;Leigh PN;Shaw CE;Miller CC
通讯作者：
Miller CC
DOI：
10.1083/jcb.126.4.1031
发表时间：
1994-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nixon RA;Paskevich PA;Sihag RK;Thayer CY
通讯作者：
Thayer CY
影响因子：
2.9
作者：
D. Watson;K. Fittro;P. Hoffman;J. Griffin
通讯作者：
D. Watson;K. Fittro;P. Hoffman;J. Griffin
DOI：
--
发表时间：
1996-03
期刊：
Zhongguo yao li xue bao = Acta pharmacologica Sinica
影响因子：
--
作者：
H. Ji;T. Y. Wang
通讯作者：
H. Ji;T. Y. Wang
DOI：
--
发表时间：
1995-09
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
L. Chen;Q. Chai;A. Zhao;X. Chai
通讯作者：
L. Chen;Q. Chai;A. Zhao;X. Chai