An Experimental Murine Model to Study Acquisition Dynamics of Tick-Borne Langat Virus in Ixodes scapularis.
An Experimental Murine Model to Study Acquisition Dynamics of Tick-Borne Langat Virus in Ixodes scapularis.
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DOI：
10.3389/fmicb.2022.849313
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发表时间：
2022
影响因子：
5.2
通讯作者：
Sultana, Hameeda
中科院分区：
文献类型：
--
作者：
Ahmed, Waqas;Rajendran, Kundave V.;Neelakanta, Girish;Sultana, Hameeda
硬蜱从水库动物身上获取几种病原体，并将它们传播给人类。开发一种动物模型来研究这些病原体分别从壁虱体内和从壁虱体内的获取/传播动力学是具有挑战性的，因为在自然界中，壁虱取食的时间更长，并且以多个脊椎动物宿主为食。为了了解病原体获取/传播的复杂性，在合适的脊椎动物宿主上建立一种成功的蜱血喂养方法是必不可少的。在这项研究中，我们提供的证据表明，小鼠模型可以成功地用于研究扁虱传播的黄病毒成员兰加特病毒(LGTV)的获取动力学。小鼠被接种了LGTV，在血液、皮肤和包括大脑在内的其他组织中显示出可检测到的病毒载量。被允许以小鼠宿主为食的幼虫和若虫都成功地获得了LGTV载量。此外，我们还发现，在蜕皮后，LGTV从幼虫经途径传播到若虫期。此外，我们注意到LGTV下调了IsSMase在所有人群中的表达，这可能是因为它在载体宿主中存活。综上所述，我们为利用小鼠模型不仅研究LGTV的获取动力学，而且还研究虫媒病毒进入硬蜱过程中Tick基因表达的变化提供了证据。壁虱传播病毒获取研究模型。
Ixodes scapularis ticks acquire several pathogens from reservoir animals and transmit them to humans. Development of an animal model to study acquisition/transmission dynamics of these pathogens into and from ticks, respectively, is challenging due to the fact that in nature ticks feed for a longer duration and on multiple vertebrate hosts. To understand the complex nature of pathogen acquisition/transmission, it is essential to set up a successful tick blood feeding method on a suitable vertebrate host. In this study, we provide evidence that murine model can be successfully used to study acquisition dynamics of Langat virus (LGTV), a member of tick-borne flaviviruses. Mice were inoculated intraperitoneally with LGTV that showed detectable viral loads in blood, skin, and other tissues including the brain. Both larval and nymphal ticks that were allowed to feed on the murine host successfully acquired LGTV loads. Also, we found that after molting, LGTV was transstadially transmitted from larval to nymphal stage. In addition, we noted that LGTV down-regulated IsSMase expression in all groups of ticks possibly for its survival in its vector host. Taken together, we provide evidence for the use of murine model to not only study acquisition dynamics of LGTV but also to study changes in tick gene expression during acquisition of arboviruses into ticks. Tick-borne virus acquisition study model.
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DOI：
10.3390/v10070362
发表时间：
2018-07-07
期刊：
Viruses
影响因子：
--
作者：
Hermance ME;Thangamani S
通讯作者：
Thangamani S
DOI：
10.1097/nen.0000000000000131
发表时间：
2014-12-01
影响因子：
3.2
作者：
Maffioli, Carola;Grandgirard, Denis;Leib, Stephen L.
通讯作者：
Leib, Stephen L.
影响因子：
3.7
作者：
Mitzel, Dana N.;Wolfinbarger, James B.;Bloom, Marshall E.
通讯作者：
Bloom, Marshall E.
影响因子：
4.5
作者：
Ramasamy, Ellango;Taank, Vikas;Neelakanta, Girish
通讯作者：
Neelakanta, Girish
影响因子：
2.2
作者：
Hart, Charles Edward;Thangamani, Saravanan
通讯作者：
Thangamani, Saravanan