Effects of chronic cocaine in rat C6 astroglial cells.
Effects of chronic cocaine in rat C6 astroglial cells.
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DOI：
10.3892/ijmm.2012.1038
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发表时间：
2012-09
影响因子：
5.4
通讯作者：
Goodman CB
中科院分区：
文献类型：
--
作者：
Badisa RB;Goodman CB
在药物滥用研究中，对星形胶质细胞的研究与对神经元的研究具有同等的重要性。本研究旨在探讨长期给予可卡因对大鼠星形胶质瘤细胞作用24小时后细胞存活率、一氧化氮(NO)生成、线粒体呼吸状态及总蛋白水平的影响。此外，还观察了可卡因对卤虫幼体的作用，以研究其对药物的敏感性。观察到可卡因引起星形胶质细胞存活率显著降低，呈剂量依赖性，半数致死浓度为4.717 mM。研究发现，可卡因不会诱导或抑制细胞内NO的产生。对星形胶质细胞线粒体脱氢酶活性的评估表明，可卡因显著干扰线粒体的一般呼吸状态，ED50值为6.153 mM。此外，可卡因可耗尽细胞中的总蛋白水平，ED50值为5.435 mM。对卤虫幼体的体内研究表明，这些幼体对可卡因高度敏感，ED50为2.41 mM。综上所述，我们的发现表明可卡因诱导的细胞毒性是非特异性的。呼吸和细胞总蛋白的显著丧失所产生的累积效应是星形胶质细胞死亡的原因。
Investigations with astroglial cells carry equal importance as those with neurons in drug abuse studies. The present study was aimed to investigate the effect of chronic cocaine administration on cell viability, nitric oxide (NO) production, general respiratory status of mitochondria and total protein levels in rat astroglioma cells after 24 h of treatment. In addition, the effect of cocaine was assessed for 24 h on brine shrimp larvae in order to study their sensitivity to the drug. It was observed that cocaine caused a significant dose-dependent decrease in astroglial cell viability with an LC50 of 4.717 mM. It was found that cocaine did not induce or inhibit NO production in the cells. Evaluation of mitochondrial dehydrogenase activity in terms of formazan production in astroglial cells indicated that cocaine significantly interfered with the general respiratory status of mitochondria with an ED50 of 6.153 mM. Furthermore, cocaine was shown to deplete the total protein levels in the cells with an ED50 of 5.435 mM. In vivo study with brine shrimp larvae showed that these larvae were highly sensitive to cocaine with an ED50 of 2.41 mM. In summary, our findings suggest that cocaine-induced cytotoxicity in the cells was non-specific. The cumulative effect arising from the significant loss of respiration and total cellular proteins is the cause of astroglial cell death.
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DOI：
10.1124/jpet.104.073494
发表时间：
2005-01-01
影响因子：
3.5
作者：
Li, GH;Xiao, YH;Zhang, LB
通讯作者：
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影响因子：
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作者：
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DOI：
10.1152/ajplung.2000.278.4.l858
发表时间：
2000-04-01
影响因子：
4.9
作者：
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通讯作者：
Saumon, G
影响因子：
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DOI：
10.1006/bbrc.1994.2716
发表时间：
1994-11-30
影响因子：
3.1
作者：
DORHEIM, MA;TRACEY, WR;GRAMMAS, P
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GRAMMAS, P