Detection of hypoxia in microscopic tumors using 131I-labeled iodo-azomycin galactopyranoside (131I-IAZGP) digital autoradiography.
Detection of hypoxia in microscopic tumors using 131I-labeled iodo-azomycin galactopyranoside (131I-IAZGP) digital autoradiography.
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DOI：
10.1007/s00259-009-1310-y
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发表时间：
2010-02
影响因子：
9.1
通讯作者：
O'Donoghue, Joseph A.
中科院分区：
文献类型：
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作者：
Li, Xiao-Feng;Sun, Xiaorong;Ma, Yuanyuan;Suehiro, Makiko;Zhang, Mutian;Russell, James;Humm, John L.;Ling, C. Clifton;O'Donoghue, Joseph A.
先前的研究已经表明，当在裸鼠中腹膜内或皮内生长时，源自HT 29结直肠癌细胞的直径小于1 mm的肿瘤是极度缺氧的，而那些更大尺寸（约1-4 mm直径）的肿瘤则不显著缺氧。本研究的目的是确定使用放射性标记的缺氧成像示踪剂碘氮霉素吡喃半乳糖苷（131 I-IAZGP）的数字放射自显影术是否可以检测到该模型中的缺氧。显微镜下HT 29肿瘤在裸鼠中生长为播散性腹膜疾病和皮内。肿瘤的大小从几百微米到直径几毫米不等。动物在处死前2 h静脉注射131 I-IAZGP和哌莫硝唑。处死后，通过数字放射自显影术评估131 I-IAZGP的肿瘤内分布，并与哌莫硝唑结合和碳酸酐酶IX（CAIX）表达的免疫荧光显微镜图像进行比较。131 I-IAZGP、哌莫硝唑和CAIX的表达分布相似。直径小于1 mm的肿瘤显示出高的131 I-IAZGP摄取;这些肿瘤也对哌莫硝唑和CAIX染色强烈。较大的肿瘤（直径约1-4 mm）没有明显的缺氧，131 I-IAZGP积累较低。131 I-IAZGP可检测显微肿瘤中的缺氧。显微肿瘤是验证乏氧放射性示踪剂的有用模型，数字放射自显影是研究乏氧放射性示踪剂在显微肿瘤中分布的适当技术。
Previous studies have shown that tumors less than 1 mm diameter derived from HT29 colorectal cancer cells are extremely hypoxic when grown intraperitoneally or intradermally in nude mice, whereas those of greater size (approximately 1–4 mm diameter) are not significantly hypoxic. The object of this study was to determine if digital autoradiography using the radiolabeled hypoxia imaging tracer iodo-azomycin galactopyranoside (131I-IAZGP) could detect hypoxia in this model. Microscopic HT29 tumors were grown as disseminated peritoneal disease and intradermally in nude mice. Tumors ranged in size from a few hundred microns to several millimeters in diameter. Animals were intravenously administered 131I-IAZGP and pimonidazole 2 h before sacrifice. Following sacrifice, the intratumoral distribution of 131I-IAZGP was assessed by digital autoradiography and compared with immunofluorescence microscopic images of pimonidazole binding and carbonic anhydrase IX (CAIX) expression. The distributions of 131I-IAZGP, pimonidazole, and CAIX expression were similar. Tumors less than 1 mm diameter displayed high 131I-IAZGP uptake; these tumors also stained strongly for pimonidazole and CAIX. Larger tumors (approximately 1–4 mm diameter) were not significantly hypoxic and had low 131I-IAZGP accumulation. 131I-IAZGP can detect hypoxia in microscopic tumors. Microscopic tumors are useful models for the validation of hypoxia radiotracers, and digital autoradiography is an appropriate technique for studying the distribution of hypoxia radiotracers in microscopic tumors.
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影响因子：
9
作者：
Driessen, A;Landuyt, W;Ectors, N
通讯作者：
Ectors, N
影响因子：
11.2
作者：
Li, Xiao-Feng;Carlin, Sean;O'Donoghue, Joseph A.
通讯作者：
O'Donoghue, Joseph A.
影响因子：
19.7
作者：
Riedl, Christopher C.;Brader, Peter;Fong, Yuman
通讯作者：
Fong, Yuman
影响因子：
8.8
作者：
通讯作者：
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影响因子：
8.8
作者：
通讯作者：
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