Heparin-binding epidermal growth factor-like growth factor protects mesenchymal stem cells.
Heparin-binding epidermal growth factor-like growth factor protects mesenchymal stem cells.
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DOI：
10.1016/j.jss.2012.05.016
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Besner GE
中科院分区：
文献类型：
--
作者：
Watkins DJ;Zhou Y;Chen CL;Darbyshire A;Besner GE
我们之前已经证明，在肠缺血/再灌注（I/R）损伤小鼠模型中，间充质干细胞（MSC）可以保护肠道免受损伤。我们还发现肝素结合的egf样生长因子（HB-EGF）是体内一种有效的肠道细胞保护剂，可能通过其对SC的作用来保护肠道。当前研究的目的是检查HB-EGF对羊水（AF）和骨髓（BM）来源的MSCs的体外影响。从泛egfp小鼠的羊水和骨髓中分离MSCs，在MSCs特异性培养基中培养，并根据其粘附特性通过顺序传代纯化。多能性通过诱导分化得到证实。将MSC与HB-EGF孵育后，使用CyQuant细胞增殖测定试剂盒在常氧和缺氧条件下定量增殖，使用CHEMICON QCM细胞迁移试剂盒定量趋化，使用caspase-3免疫组织化学检测MSC缺氧应激后的凋亡。AF-MSC和BM-MSC对HB-EGF的增殖和迁移反应显著增加。HB-EGF显著保护AF-MSC和BM-MSC免于缺氧诱导的细胞凋亡。HB-EGF在AF-MSC中的增殖和抗凋亡作用比BM-MSC更明显。这些结果表明HB-EGF作为MSC的有丝分裂和趋化剂，可以保护MSC免受损伤。这些发现可能对未来设计利用MSC保护肠道免受损伤的实验具有重要意义。
We have previously demonstrated that mesenchymal stem cell (MSC) administration protects the intestines from injury in a mouse model of intestinal ischemia/reperfusion (I/R) injury. We have also shown that heparin-binding EGF-like growth factor (HB-EGF) is a potent intestinal cytoprotective agent in vivo that may protect the intestines via its effects on SC (SC). The goal of the current study was to examine the effects of HB-EGF on both amniotic fluid (AF)- and bone marrow (BM)-derived MSCs in vitro. MSCs were isolated from the amniotic fluid and bone marrow of pan-EGFP mice, grown in MSC-specific culture medium, and purified by sequential passages based on their adherence properties. Pluripotency was confirmed by induced differentiation. After incubation of MSCs with HB-EGF, proliferation was quantified using the CyQuant cell proliferation assay kit under normoxic and anoxic conditions, chemotaxis was quantified using the CHEMICON QCM cell migration kit, and apoptosis was determined by caspase-3 immunohistochemistry after exposure of MSC to anoxic stress. AF-MSC and BM-MSC showed significantly increased proliferation and migration in response to HB-EGF. HB-EGF significantly protected AF-MSC and BM-MSC from anoxia-induced apoptosis. The proliferative and anti-apoptotic effects of HB-EGF were even more pronounced in AF-MSC compared with BM-MSC. These results demonstrate that HB-EGF acts as a mitogenic and chemotactic agent for MSC that protects MSC from injury. These findings may have important implications for future experiments designed to utilize MSC to protect the intestines from injury.
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DOI：
10.1038/labinvest.2011.167
发表时间：
2012-03
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1091/mbc.1.11.811
发表时间：
1990-10-01
期刊：
CELL REGULATION
影响因子：
--
作者：
BESNER, G;HIGASHIYAMA, S;KLAGSBRUN, M
通讯作者：
KLAGSBRUN, M
影响因子：
20.3
作者：
Kawada, H;Fujita, J;Fukuda, K
通讯作者：
Fukuda, K
影响因子：
1.7
作者：
Koda, M;Okada, S;Yamazaki, M
通讯作者：
Yamazaki, M
影响因子：
1.2
作者：
Peister, Alexandra;Woodruff, Maria A.;Prince, Jarod J.;Gray, Derwin P.;Hutmacher, Dietmar W.;Guldberg, Robert E.
通讯作者：
Guldberg, Robert E.