Dynamic changes in hair cell stereocilia and cochlear transduction after noise exposure.
Dynamic changes in hair cell stereocilia and cochlear transduction after noise exposure.
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噪声暴露后毛细胞静纤毛和耳蜗转导的动态变化。
DOI：
10.1016/j.bbrc.2011.05.049
复制
发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
殷善开
中科院分区：
文献类型：
--
作者：
殷善开
耳蜗传导的结构包括毛细胞顶面的静纤毛及其与盖膜的连接。传导部位是噪声性耳蜗损伤的部位之一。虽然静纤毛对噪声敏感，但已经发现，在鸟类的内耳中，这种脆弱的结构在很大程度上是自我修复的，并且与噪声暴露后听力灵敏度的恢复有关，如在时间阈值偏移（TTS）和永久阈值偏移（PTS）之间的差异中所观察到的。然而，在哺乳动物耳蜗，在听觉脑干反应（ABR）中测得的阈值偏移并不平行的毛细胞上的静纤毛的时间变化。目前还不清楚哺乳动物毛细胞上静纤毛的形态恢复如何与耳蜗转导的变化相关，耳蜗转导的变化可以通过测量受体电位来评估。在本研究中，豚鼠暴露于110 dB SPL的宽带噪声中2 h。听觉灵敏度采用ABR评估，耳蜗传导采用耳蜗微音电位（CM）评估。在噪声后的不同时间点对静纤毛形态进行定量，并与对照组进行比较。噪声产生的TTS频率平均ABR阈值为55.69 ± 14.13dB。1个月后阈移降至9.58 ± 11.75dBSPL，毛细胞几乎无损失。噪声暴露后立即对静纤毛的损伤与CM振幅的降低有关。噪音后1个月，几乎没有观察到与完全恢复的CM相关的异常静纤毛。
The structures of cochlear transduction include stereocilia at the apical surface of hair cells and their connection to the tectorial membrane. The transduction site is one of the loci for noise-induced cochlear damage. Although stereocilia are susceptible to noise, it has been found that in the inner ears of avians, this fragile structure is largely self-repairing and is associated with recovery of hearing sensitivity after noise exposure, as observed in the difference between the temporal threshold shift (TTS) and the permanent threshold shift (PTS). In the mammalian cochleae, however, threshold shifts measured in the auditory brainstem responses (ABR) did not parallel the chronological changes in the stereocilia on hair cells. It is unclear how the morphological recovery of the stereocilia on the mammalian hair cells is correlated with the changes in cochlear transduction that can be assessed by measuring receptor potential. In the present study, guinea pigs were exposed to a broadband noise of 110 dB SPL for 2 h. Auditory sensitivity was evaluated using ABR and cochlear transduction was assessed using cochlear microphonics (CM). Stereocilia morphology was quantified at different time points after the noise and compared with the control. The noise produced a TTS of 55.69 ± 14.13 dB in frequency-averaged ABR thresholds. The threshold shift was reduced to 9.58 ± 11.75 dB SPL 1 month later with virtually no loss of hair cells. Damage to the stereocilia immediately after noise exposure was found to be associated with depression of CM amplitude. Virtually no abnormal stereocilia were observed 1 month after the noise in association with a fully recovered CM.
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DOI：
10.1121/1.1912810
发表时间：
1971-12
期刊：
The Journal of the Acoustical Society of America
影响因子：
--
作者：
P. Dallos
通讯作者：
P. Dallos
DOI：
10.1002/neu.10002
发表时间：
2002-02-05
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Gale, JE;Meyers, JR;Corwin, JT
通讯作者：
Corwin, JT
DOI：
10.1073/pnas.97.22.11722
发表时间：
2000-10-24
影响因子：
11.1
作者：
Baird, RA;Burton, MD;Naeger, RA
通讯作者：
Naeger, RA
DOI：
10.1023/b:neur.0000021906.08847.d2
发表时间：
2003-11
期刊：
Journal of Neurocytology
影响因子：
--
作者：
V. Tsuprun;P. Schachern;Sebahattin Cureoglu;M. Paparella
通讯作者：
V. Tsuprun;P. Schachern;Sebahattin Cureoglu;M. Paparella
影响因子：
2.8
作者：
G. W. Harding;P. J. Baggot;B. Bohne
通讯作者：
G. W. Harding;P. J. Baggot;B. Bohne