Bacterial protein HU dictates the morphology of DNA condensates produced by crowding agents and polyamines.
Bacterial protein HU dictates the morphology of DNA condensates produced by crowding agents and polyamines.
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DOI：
10.1093/nar/gkl1093
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发表时间：
2007
影响因子：
14.9
通讯作者：
Hud NV
中科院分区：
文献类型：
--
作者：
Sarkar T;Vitoc I;Mukerji I;Hud NV
在体内，控制DNA凝聚体的大小和形状对于改善非病毒基因的传递非常重要。在这里，我们证明了在各种条件下形成的DNA凝聚体的形态完全从环状转变为杆状，如果细菌蛋白HU在缩合过程中存在。HU是一种非序列特异性DNA结合蛋白，它能使DNA急剧弯曲，但单独不能将DNA浓缩成紧密堆积的颗粒。当DNA被亚精胺缩合时，每225bp的DNA中不到一个HU二聚体就足以完全控制凝聚体的形态。我们认为，在HU存在的情况下，棒是受欢迎的，因为棒包含急剧弯曲的DNA，而环状DNA只包含平滑弯曲的DNA。这些结果说明了天然提取的蛋白质在控制体外形成的DNA凝聚体形状方面的效用。HU是细菌中的一种高度保守的蛋白质，与类核结构的致密和形成有关。然而，HU在染色体紧凑中的确切作用还不是很清楚。我们的研究表明，HU在体外控制DNA凝聚，这也表明了HU在体内可以作为细菌染色体紧凑和组织的建筑蛋白。
Controlling the size and shape of DNA condensates is important in vivo and for the improvement of nonviral gene delivery. Here, we demonstrate that the morphology of DNA condensates, formed under a variety of conditions, is shifted completely from toroids to rods if the bacterial protein HU is present during condensation. HU is a non-sequence-specific DNA binding protein that sharply bends DNA, but alone does not condense DNA into densely packed particles. Less than one HU dimer per 225 bp of DNA is sufficient to completely control condensate morphology when DNA is condensed by spermidine. We propose that rods are favored in the presence of HU because rods contain sharply bent DNA, whereas toroids contain only smoothly bent DNA. The results presented illustrate the utility of naturally derived proteins for controlling the shape of DNA condensates formed in vitro. HU is a highly conserved protein in bacteria that is implicated in the compaction and shaping of nucleoid structure. However, the exact role of HU in chromosome compaction is not well understood. Our demonstration that HU governs DNA condensation in vitro also suggests a mechanism by which HU could act as an architectural protein for bacterial chromosome compaction and organization in vivo.
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影响因子：
3.2
作者：
HILLYARD, DR;EDLUND, M;HIGGINS, NP
通讯作者：
HIGGINS, NP
影响因子：
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作者：
ARSCOTT, PG;MA, CL;BLOOMFIELD, VA
通讯作者：
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DOI：
10.1002/(sici)1098-2795(200006)56:2
发表时间：
2000-06-01
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作者：
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通讯作者：
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