Electro-Acupuncture Alleviates Chronic Unpredictable Stress-Induced Depressive- and Anxiety-Like Behavior and Hippocampal Neuroinflammation in Rat Model of Depression.
Electro-Acupuncture Alleviates Chronic Unpredictable Stress-Induced Depressive- and Anxiety-Like Behavior and Hippocampal Neuroinflammation in Rat Model of Depression.
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电针缓解抑郁症大鼠模型中慢性不可预测的压力引起的抑郁和焦虑样行为以及海马神经炎症
DOI：
10.3389/fnmol.2018.00149
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发表时间：
2018
影响因子：
4.8
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Yue N;Li B;Yang L;Han QQ;Huang HJ;Wang YL;Wang J;Yu R;Wu GC;Liu Q;Yu J
抑郁症是全球第二大致残原因。临床抑郁症的影响可能是由神经炎症介导的，如小胶质细胞的激活和某些脑区高水平的促炎细胞因子。在亚洲，电针(EA)等传统中医技术被广泛用于治疗精神健康障碍。然而，电针治疗抑郁症的研究还不是很严格。本研究旨在评价电针治疗抑郁样行为的有效性，并探讨海马神经炎症在电针抗抑郁作用中的作用。在这项研究中，我们以随机顺序每天使用六种慢性不可预测的应激源，持续10周。电针“百会”(杜-20)(+)、“阳陵泉”(GB-34，右侧；−)，采用电针治疗仪(汉斯电子仪，LH202H型，2/100 Hz，0.3 mA)，隔日1次，每次30min，连续4周。在第11周的周一和周二进行行为测试，包括旷场测试和强迫游泳测试，这两项测试广泛用于评估抑郁和焦虑样行为。结果表明，10周的慢性不可预知应激可导致大鼠行为障碍和海马神经炎症，表现为NLRP3炎症体成分表达增加，IL-1β表达上调，IL-1β成熟蛋白(P17)蛋白水平升高，小胶质细胞活化。此外，4周的电针治疗可显著减轻CUS所致的行为障碍。电针的抗抑郁作用伴随着某些炎症体成分NLRP3的表达和成熟的IL-1β的显著降低。同时，电针治疗可明显逆转CUS诱导的P2X7受体、Iba-1、IL-18、肿瘤坏死因子α和IL-6的表达增加，降低胶质纤维酸性蛋白的表达。综上所述，电针具有抗抑郁作用，并能减轻海马神经炎症。这些发现可能有助于深入了解海马神经炎症在电针抗抑郁作用中的作用。
Depression is the second leading cause of disability worldwide. The effects of clinical depression may be mediated by neuroinflammation such as activation of microglia and high levels of proinflammatory cytokines in certain brain areas. Traditional Chinese medicine techniques such as electro-acupuncture (EA) are used extensively in Asia to treat mental health disorders. However, EA has not been rigorously studied in treatment of depression. This study was designed to assess the effectiveness of EA on depressive-like behavior and explore the role of hippocampal neuroinflammation in the potential antidepressant effect of EA. In this study, we used six chronic unpredictable stressors daily in a random sequence for 10 weeks. EA were performed on “Bai-Hui” (Du-20) (+) and “Yang-Ling-Quan” (GB-34, the right side; −) acupoints by an EA apparatus (HANS Electronic Apparatus, LH202H, 2/100 Hz, 0.3 mA) for 30 min once every other day for last 4 weeks. The behavior tests including open field test and forced swimming test, which are widely used to assess depressive and anxiety-like behavior were performed on the Monday and Tuesday of the eleventh week. The results showed that 10 week of chronic unpredictable stress (CUS) caused behavioral deficits in rats and neuroinflammation in hippocampus, such as increased expression of NLRP3 inflammasome components, upregulated mRNA level of IL-1β and the protein level of IL-1β mature form (p17) and activation of microglia. Moreover, 4 weeks of EA treatment significantly attenuated behavioral deficits caused by CUS. EA’s antidepressant effect was accompanied by markedly decreased expression of certain NLRP3 inflammasome components and matured IL-1β. Meanwhile, EA treatment can significantly reverse CUS-induced increases in P2X7 receptor, Iba-1, IL-18, TNFα and IL-6 expression and decreases in GFAP expression. In conclusion, EA exhibited the antidepressant effect and alleviated the hippocampal neuroinflammation. These findings may provide insight into the role of hippocampal neuroinflammation in the antidepressant effect of EA.
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影响因子：
16.8
作者：
Hanamsagar R;Hanke ML;Kielian T
通讯作者：
Kielian T
影响因子：
2.7
作者：
Hazra S;Kumar S;Saha GK;Mondal AC
通讯作者：
Mondal AC
影响因子：
2.9
作者：
Brown, Guy C.;Vilalta, Anna
通讯作者：
Vilalta, Anna
DOI：
10.1136/acupmed-2015-010981
发表时间：
2016-10
期刊：
Acupuncture in medicine : journal of the British Medical Acupuncture Society
影响因子：
--
作者：
Guo T;Guo Z;Zhang W;Ma W;Yang X;Yang X;Hwang J;He X;Chen X;Ya T
通讯作者：
Ya T
影响因子：
3.7
作者：
Gustin A;Kirchmeyer M;Koncina E;Felten P;Losciuto S;Heurtaux T;Tardivel A;Heuschling P;Dostert C
通讯作者：
Dostert C