Oxidized phosphatidylserine-CD36 interactions play an essential role in macrophage-dependent phagocytosis of apoptotic cells.
Oxidized phosphatidylserine-CD36 interactions play an essential role in macrophage-dependent phagocytosis of apoptotic cells.
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DOI：
10.1084/jem.20060370
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发表时间：
2006-11-27
期刊：
影响因子：
--
通讯作者：
Hazen SL
中科院分区：
文献类型：
--
作者：
Greenberg ME;Sun M;Zhang R;Febbraio M;Silverstein R;Hazen SL
凋亡细胞的吞噬作用是细胞程序性死亡的重要终末生理过程。有证据表明，巨噬细胞的凋亡细胞吞噬和清除是促进磷脂酰丝氨酸（PS）显示在外表面的质膜;然而，无论是负责PS识别的巨噬细胞受体，也没有表征的PS分子物种可能涉及，已明确定义。我们发现，B类清道夫受体CD 36在体内巨噬细胞清除凋亡细胞中起着重要作用。此外，巨噬细胞通过CD 36识别凋亡细胞显示通过与膜相关的氧化PS（oxPS）的相互作用发生，并在较小程度上，氧化磷脂酰胆碱，但不是非氧化PS分子种类。oxPS物质的质谱分析鉴定了凋亡膜中可能促进巨噬细胞识别的CD 36候选配体的结构。总的来说，这些结果确定巨噬细胞上的oxPS-CD 36相互作用作为涉及巨噬细胞介导的凋亡细胞吞噬的广泛生理过程中的潜在参与者。
The phagocytosis of apoptotic cells within an organism is a critical terminal physiological process in programmed cell death. Evidence suggests that apoptotic cell engulfment and removal by macrophages is facilitated by phosphatidylserine (PS) displayed at the exofacial surface of the plasma membrane; however, neither the macrophage receptors responsible for PS recognition, nor characterization of the PS molecular species potentially involved, have been clearly defined. We show that the class B scavenger receptor CD36 plays an essential role in macrophage clearance of apoptotic cells in vivo. Further, macrophage recognition of apoptotic cells via CD36 is shown to occur via interactions with membrane-associated oxidized PS (oxPS) and, to a lesser extent, oxidized phosphatidylcholine, but not nonoxidized PS molecular species. Mass spectrometry analyses of oxPS species identify structures of candidate ligands for CD36 in apoptotic membranes that may facilitate macrophage recognition. Collectively, these results identify oxPS–CD36 interactions on macrophages as potential participants in a broad range of physiologic processes where macrophage-mediated engulfment of apoptotic cells is involved.
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DOI：
10.1084/jem.194.9.1289
发表时间：
2001-11-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Finnemann SC;Silverstein RL
通讯作者：
Silverstein RL
影响因子：
4.8
作者：
RIGOTTI, A;ACTON, SL;KRIEGER, M
通讯作者：
KRIEGER, M
DOI：
10.1097/00075197-200203000-00004
发表时间：
2002-03-01
影响因子：
3.1
作者：
Ibrahimi, A;Abumrad, NA
通讯作者：
Abumrad, NA
影响因子：
15.9
作者：
Podrez, EA;Schmitt, D;Hazen, SL
通讯作者：
Hazen, SL
影响因子：
4.8
作者：
Febbraio, M;Abumrad, NA;Silverstein, RL
通讯作者：
Silverstein, RL