Biomimetic postcapillary expansions for enhancing rare blood cell separation on a microfluidic chip.
Biomimetic postcapillary expansions for enhancing rare blood cell separation on a microfluidic chip.
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DOI：
10.1039/c1lc20401g
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发表时间：
2011-09-07
期刊：
影响因子：
6.1
通讯作者：
Munn LL
中科院分区：
文献类型：
--
作者：
Jain A;Munn LL
血细胞在毛细血管后小静脉中自然自动分离，红细胞(红细胞，RBC)聚集在血流轴附近，有核细胞(NC)--包括白细胞、祖细胞和癌症患者循环中的肿瘤细胞--向血管壁边缘聚集。我们利用这一原理设计了一种从全血中提取有核细胞(NC)的微流控装置。该仿生细胞提取装置采用聚二甲基硅氧烷软光刻技术制作，由20-400μm宽、11μm深、长达2 cm的矩形微通道组成。其主要设计特点是使用了模仿毛细血管后小静脉的三角形几何重复扩张/收缩，从而增强了边缘并优化了提取。该设备对未经处理的全血进行操作，能够以5nL S−1的速度提取94±4.5%的神经干细胞，浓缩倍数为45.75±2.5倍。该设备不需要通过离心法或红细胞裂解对血液进行前处理，可作为需要浓缩有核细胞的芯片实验室的初始阶段实施。潜在的下游应用有很多，包括对浓缩的NC人群进行操作的所有临床前和临床分析，包括芯片上的流式细胞术
Blood cells naturally auto-segregate in postcapillary venules, with the erythrocytes (red blood cells, RBCs) aggregating near the axis of flow and the nucleated cells (NCs)—which include leukocytes, progenitor cells and, in cancer patients, circulating tumor cells—marginating toward the vessel wall. We have used this principle to design a microfluidic device that extracts nucleated cells (NCs) from whole blood. Fabricated using polydimethylsiloxane (PDMS) soft lithography, the biomimetic cell extraction device consists of rectangular microchannels that are 20–400 μm wide, 11 μm deep and up to 2 cm long. The key design feature is the use of repeated expansions/contractions of triangular geometry mimicking postcapillary venules, which enhance margination and optimize the extraction. The device operates on unprocessed whole blood and is able to extract 94 ± 4.5% of NCs with 45.75 ± 2.5-fold enrichment in concentration at a rate of 5 nl s−1. The device eliminates the need to preprocess blood via centrifugation or RBC lysis, and is ready to be implemented as the initial stage of lab-on-a-chip devices that require enriched nucleated cells. The potential downstream applications are numerous, encompassing all preclinical and clinical assays that operate on enriched NC populations and include on-chip flow cytometry
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影响因子：
3.7
作者：
Jain A;Munn LL
通讯作者：
Munn LL
影响因子：
50.5
作者：
Beerepoot, LV;Mehra, N;Voest, EE
通讯作者：
Voest, EE
影响因子：
9.7
作者：
Beerepoot, LV;Mehra, N;Voest, EE
通讯作者：
Voest, EE
影响因子：
--
作者：
Del Papa, N;Colombo, G;Cortelezzi, A
通讯作者：
Cortelezzi, A
影响因子：
2.9
作者：
Humpe, A;Beck, C;Horst, HA
通讯作者：
Horst, HA