Artificial lymphatic drainage systems for vascularized microfluidic scaffolds.
Artificial lymphatic drainage systems for vascularized microfluidic scaffolds.
复制标题
DOI：
10.1002/jbm.a.34524
复制
发表时间：
2013-08
影响因子：
4.9
通讯作者：
Tien, Joe
中科院分区：
文献类型：
--
作者：
Wong, Keith H. K.;Truslow, James G.;Khankhel, Aimal H.;Chan, Kelvin L. S.;Tien, Joe
关键词：
形成稳定灌注的微血管系统一直是组织工程的主要挑战。先前的研究表明足够大的跨壁压力对维持血管稳定和灌注的重要性。在这里，我们展示了一个提供类似于体内淋巴微血管引流功能的空通道系统，可以稳定血管粘连并维持致密的体外水力阻力纤维蛋白支架的灌注率。在没有引流的情况下，随着支架密度从6 mg/mL增加到30 mg/mL，内皮层脱层增加，支架的水力导电性降低了20倍。单一引流通道仅发挥局部血管稳定作用，其程度取决于血管与引流管之间的距离以及支架密度。这些实验的计算模型估计血管稳定所需的最小跨壁压力为0.40-1.36 cm H2O。我们进一步设计和构建了纤维蛋白贴片（0.8 × 0.9 cm2），由平行排列的血管灌注，并通过正交排列的排水通道排出；只有引流才能使血管表现出长期的稳定和灌注。这项工作强调了引流在血管化中的重要性，特别是当使用致密的水力阻力支架时。
The formation of a stably perfused microvasculature continues to be a major challenge in tissue engineering. Previous work has suggested the importance of a sufficiently large transmural pressure in maintaining vascular stability and perfusion. Here we show that a system of empty channels that provides a drainage function analogous to that of lymphatic microvasculature in vivo can stabilize vascular adhesion and maintain perfusion rate in dense, hydraulically resistive fibrin scaffolds in vitro. In the absence of drainage, endothelial delamination increased as scaffold density increased from 6 mg/mL to 30 mg/mL and scaffold hydraulic conductivity decreased by a factor of twenty. Single drainage channels exerted only localized vascular stabilization, the extent of which depended on the distance between vessel and drainage as well as scaffold density. Computational modeling of these experiments yielded an estimate of 0.40–1.36 cm H2O for the minimum transmural pressure required for vascular stability. We further designed and constructed fibrin patches (0.8 by 0.9 cm2) that were perfused by a parallel array of vessels and drained by an orthogonal array of drainage channels; only with the drainage did the vessels display long-term stability and perfusion. This work underscores the importance of drainage in vascularization, especially when a dense, hydraulically resistive scaffold is used.
登录
查看更多内容
影响因子：
14
作者：
Cuchiara, Michael P.;Allen, Alicia C. B.;West, Jennifer L.
通讯作者：
West, Jennifer L.
影响因子：
3.8
作者：
LEE, J;SCHMIDSCHONBEIN, GW
通讯作者：
SCHMIDSCHONBEIN, GW
影响因子：
6.1
作者：
Ling, Yibo;Rubin, Jamie;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
15
作者：
Price, Gavrielle M.;Chu, Kengyeh K.;Tien, Joe
通讯作者：
Tien, Joe
影响因子：
3.1
作者：
Price, Gavrielle M.;Chrobak, Kenneth M.;Tien, Joe
通讯作者：
Tien, Joe