Comparison of Autologous Blood Clots with Fibrin Sealant as Scaffolds for Promoting Human Muscle-Derived Stem Cell-Mediated Bone Regeneration.
Comparison of Autologous Blood Clots with Fibrin Sealant as Scaffolds for Promoting Human Muscle-Derived Stem Cell-Mediated Bone Regeneration.
复制标题
自体血凝块与纤维蛋白密封胶作为促进肌源性干细胞介导的骨再生的支架的比较。
DOI：
10.3390/biomedicines9080983
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发表时间：
2021-08-09
期刊：
影响因子：
4.7
通讯作者：
Huard J
中科院分区：
文献类型：
--
作者：
Gao X;Cheng H;Sun X;Lu A;Ruzbarsky JJ;Wang B;Huard J
背景骨密封剂已被用作支架来递送用于骨再生的遗传修饰的人肌源性干细胞（hMDSC）。或者，自体血凝块是安全、经济的支架。本研究比较了自体血凝块（BC）与纤维蛋白封闭剂（FS）作为支架递送用于骨再生的lenti-BMP 2/GFP转导的hMDSC。方法.体外成骨分化使用3D小球培养进行，并使用microCT和Von Kossa染色进行评价。然后将lenti-GFP转导的细胞与人血液混合以评价成骨分化。此外，利用鼠临界尺寸的颅骨缺损模型来比较BC和FS支架用于慢BMP 2/GFP转导的hMDSC介导的骨再生，并用显微CT和组织学进行评价。结果慢-BMP 2/GFP转导的hMDSC形成比未转导的hMDSC显著更大的矿化团块。人血凝块内的hMDSC迁移出来并分化为ALP+成骨细胞。在体内，BC导致在临界大小的颅骨骨缺损内的新骨形成比FS支架显著更少，尽管在新形成的骨中没有观察到GFP+供体细胞、破骨细胞和成骨细胞的差异。结论.人lenti-BMP 2/GFP转导的hMDSCs可以在体外有效地进行成骨分化。出乎意料的是，BC组的新再生骨明显少于FS组。自体血凝块支架在递送干细胞用于骨再生方面不如纤维蛋白密封剂有效。
Background. Fibrin sealant has been used as a scaffold to deliver genetically modified human muscle-derived stem cells (hMDSCs) for bone regeneration. Alternatively, autologous blood clots are safe, economic scaffolds. This study compared autologous blood clot (BC) with fibrin sealant (FS) as a scaffold to deliver lenti-BMP2/GFP-transduced hMDSCs for bone regeneration. Methods. In vitro osteogenic differentiation was performed using 3D pellet culture and evaluated using microCT and Von Kossa staining. The lenti-GFP transduced cells were then mixed with human blood for evaluation of osteogenic differentiation. Furthermore, a murine critical- sized calvarial defect model was utilized to compare BC and FS scaffolds for lenti-BMP2/GFP-transduced hMDSCs mediated bone regeneration and evaluated with micro-CT and histology. Results. Lenti-BMP2/GFP transduced hMDSCs formed significantly larger mineralized pellets than non-transduced hMDSCs. hMDSCs within the human blood clot migrated out and differentiated into ALP+ osteoblasts. In vivo, BC resulted in significantly less new bone formation within a critical-sized calvarial bone defect than FS scaffold, despite no difference observed for GFP+ donor cells, osteoclasts, and osteoblasts in the newly formed bone. Conclusions. Human lenti-BMP2/GFP-transduced hMDSCs can efficiently undergo osteogenic differentiation in vitro. Unexpectedly, the newly regenerated bone in BC group was significantly less than the FS group. The autologous blood clot scaffold is less efficacious for delivering stem cells for bone regeneration than fibrin sealant.
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影响因子：
14
作者：
MULLERMAI, C;VOIGT, C;GROSS, UM
通讯作者：
GROSS, UM
影响因子：
4.9
作者：
Paul, Adrien J.;Momier, David;Rochet, Nathalie
通讯作者：
Rochet, Nathalie
影响因子：
4.3
作者：
Kim, Ha-Rang;Choi, Byung-Ho;Jeong, Seung-Mi
通讯作者：
Jeong, Seung-Mi
影响因子：
4.8
作者：
Gao, Xueqin;Usas, Arvydas;Huard, Johnny
通讯作者：
Huard, Johnny
影响因子：
14
作者：
Gao, Xueqin;Usas, Arvydas;Tang, Ying;Lu, Aiping;Tan, Jian;Schneppendahl, Johannes;Kozemchak, Adam M.;Wang, Bing;Cummins, James H.;Tuan, Rocky S.;Huard, Johnny
通讯作者：
Huard, Johnny