Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus Bordetella Analyzed Exclusively by NMR Spectroscopy.
Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus Bordetella Analyzed Exclusively by NMR Spectroscopy.
复制标题
核心寡糖的保守结构特征来自Bordetella属的呼吸道病原体的脂多糖，仅通过NMR光谱法分析。
DOI：
10.3390/ijms22031029
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发表时间：
2021-01-21
影响因子：
5.6
通讯作者：
Niedziela T
中科院分区：
文献类型：
--
作者：
Ucieklak K;Koj S;Niedziela T
细菌病原体在细胞表面暴露出各种复杂的碳水化合物分子。革兰氏阴性菌产生脂多糖，脂多糖是细菌外膜的主要成分，在宿主与病原菌的相互作用中起着重要作用。百日咳杆菌、副百日咳杆菌、败血杆菌和霍尔梅氏杆菌是哺乳动物呼吸道病原体，对人类健康和农业生产具有重要的经济影响。百日咳是百日咳的罪魁祸首，霍尔梅西杆菌是第二大百日咳病因，但目前的抗百日咳疫苗不能提供交叉保护。任何给定的假想碳水化合物抗原的结构数据是进一步分析结构相关活性及其与宿主相互作用的先决条件。核磁共振氢谱构成了所分析的多聚糖的指纹图谱，并提供了唯一的鉴定信息。波尔德氏菌低聚糖的~1H，~(13)C相关谱扩大了结构报告基团的概念。波氏寡糖(OS)的比较分析表明，α-GlcpN、α-GlcpA、4，6-二取代-β-Glcp、2，7-二取代-L-α-d-HEPP、3，4-二取代-L-α-d-HEPP和Kdo组成的六糖是变异最小的OS片段。波氏杆菌脂多糖结构中的这种最小公共元件可用于设计一种通用的交叉保护疫苗成分，以对抗来自波氏杆菌属的各种细菌的感染。
Bacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host–pathogen interactions. B. pertussis, B. parapertussis, B. bronchiseptica, and B. holmesii, are mammalian respiratory pathogens, having substantial economic impact on human health and agriculture. B. pertussis is responsible for whooping cough (pertussis) and B. holmesii is the second pertussis etiological factor, but the current anti-pertussis vaccines do not provide cross-protection. The structural data on any given hypothetical carbohydrate antigen is a prerequisite for further analysis of structure-related activities and their interaction with hosts. 1H NMR spectra constitute fingerprints of the analyzed glycans and provide unique identity information. The concept of structure-reporter groups has now been augmented by 1H,13C-correlation spectra of the Bordetella oligosaccharides. The comparative analysis of Bordetellae oligosaccharides (OS) revealed that the hexasaccharide, comprising the α-GlcpN, α-GlcpA, 4,6-disubstituted-β-Glcp, 2,7-disubstituted-l-α-d-Hepp, 3,4-disubstituted-l-α-d-Hepp, and Kdo, constitute the least variable OS segment. This minimal common element in the structure of lipopolysaccharides of Bordetellae could be used to devise a universal cross-protective vaccine component against infections with various bacteria from the genus Bordetella.
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影响因子：
--
作者：
Lukasiewicz, J;Jachymek, W;Lugowski, C
通讯作者：
Lugowski, C
影响因子：
15.9
作者：
Antunes, Ricardo da Silva;Babor, Mariana;Sette, Alessandro
通讯作者：
Sette, Alessandro
影响因子：
3.1
作者：
Clark, Howard W.;Mackay, Rose-Marie;Shrive, Annette K.
通讯作者：
Shrive, Annette K.
影响因子：
3.1
作者：
Buboltz, Anne M.;Nicholson, Tracy L.;Harvill, Eric T.
通讯作者：
Harvill, Eric T.
影响因子：
4.8
作者：
Preston, Andrew;Petersen, Bent O.;Vinogradov, Evgeny
通讯作者：
Vinogradov, Evgeny