Rv3091, An Extracellular Patatin-Like Phospholipase in Mycobacterium tuberculosis, Prolongs Intracellular Survival of Recombinant Mycolicibacterium smegmatis by Mediating Phagosomal Escape.
Rv3091, An Extracellular Patatin-Like Phospholipase in Mycobacterium tuberculosis, Prolongs Intracellular Survival of Recombinant Mycolicibacterium smegmatis by Mediating Phagosomal Escape.
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Rv3091，结核分枝杆菌中的一种细胞外 Patatin 样磷脂酶，通过介导吞噬体逃逸延长重组耻垢分枝杆菌的细胞内存活
DOI：
10.3389/fmicb.2020.532371
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发表时间：
2020
影响因子：
5.2
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Cui Z;Dang G;Song N;Cui Y;Li Z;Zang X;Liu H;Wang Z;Liu S
Patatin-like phospholipases（PLPs）是许多病原菌的重要毒力因子。然而，关于PLPs作为结核分枝杆菌（Mtb）毒力因子的研究还不多见。Rv 3091是Mtb的一个推定蛋白，分析表明它属于PLPs家族。在这里，我们克隆和表达rv 3091基因在耻垢分枝杆菌，随后，进行蛋白纯化和表征。我们表明，它具有磷脂酶A1，磷脂酶A2和脂肪酶活性。我们确认推定的活性位点残基，即，Ser 214和Asp 407，使用定点诱变。Rv 3091是一种胞外蛋白，可改变M.的殖民地形态。恶臭Rv 3091的存在增强了M.小鼠腹腔巨噬细胞中的耻垢病。此外，它还促进了M。巨噬细胞吞噬体逃逸。此外，Rv 3091显著增加了M. C57 BL/6 J小鼠肺内的肺水肿和加重的病变。综上所述，我们的结果表明，Rv 3091作为细胞外PLP对分枝杆菌的致病性至关重要，因为它允许分枝杆菌利用磷脂用于其生长并提供对吞噬体杀伤的抗性，从而导致其增强的细胞内存活。
Patatin-like phospholipases (PLPs) are important virulence factors of many pathogens. However, there are no prevailing studies regarding PLPs as a virulence factor of Mycobacterium tuberculosis (Mtb). Analysis of Rv3091, a putative protein of Mtb, shows that it belongs to the PLPs family. Here, we cloned and expressed the rv3091 gene in Mycobacterium smegmatis and, subsequently, conducted protein purification and characterization. We show that it possesses phospholipase A1, phospholipase A2, and lipase activity. We confirm the putative active site residues, namely, Ser214 and Asp407, using site directed mutagenesis. The Rv3091 is an extracellular protein that alters the colony morphology of M. smegmatis. The presence of Rv3091 enhances the intracellular survival capability of M. smegmatis in murine peritoneal macrophages. Additionally, it promotes M. smegmatis phagosomal escape from macrophages. Moreover, Rv3091 significantly increased the survival of M. smegmatis and aggravated lesions in C57BL/6 J murine lungs in vivo. Taken together, our results indicate that Rv3091 as an extracellular PLP that is critical to the pathogenicity of mycobacterium as it allows mycobacterium to utilize phospholipids for its growth and provides resistance to phagosome killing, resulting in its enhanced intracellular survival.
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影响因子：
7.8
作者：
Dey B;Bishai WR
通讯作者：
Bishai WR
DOI：
10.1016/j.bbalip.2012.07.014
发表时间：
2012-11-01
影响因子：
4.8
作者：
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影响因子：
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通讯作者：
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影响因子：
5.2
作者：
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通讯作者：
Son J
影响因子：
7.3
作者：
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通讯作者：
Liu S