The Bacterial Species Campylobacter jejuni Induce Diverse Innate Immune Responses in Human and Avian Intestinal Epithelial Cells.
The Bacterial Species Campylobacter jejuni Induce Diverse Innate Immune Responses in Human and Avian Intestinal Epithelial Cells.
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DOI：
10.3389/fmicb.2017.01840
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发表时间：
2017
影响因子：
5.2
通讯作者：
Wilkinson TS
中科院分区：
文献类型：
--
作者：
John DA;Williams LK;Kanamarlapudi V;Humphrey TJ;Wilkinson TS
在发达国家，弯曲杆菌仍然是人类胃肠炎的主要原因，给卫生服务造成重大负担。弯曲杆菌是人类和鸡的病原体，尽管在机理理解上的差异是不完整的，部分原因是表型菌株多样性产生了不一致的发现。在这里，我们从多位点序列分型的集合中获得空肠弯曲杆菌分离株（n = 100），通过使用禽类和人类肠上皮细胞的高通量模型中的炎症、细胞毒性、粘附、侵袭和信号传导反应来评估其致病多样性。C.空肠诱导IL-8和CXCLi 1/2在人类和鸟类上皮细胞中，分别在MAP激酶依赖的方式。相反，两种细胞类型中的IL-10应答均为PI 3-激酶/Akt依赖性。C.空肠菌株在两种细胞系中显示不同水平的侵袭，其中高侵袭依赖于MAP激酶信号传导。C.空肠在两种细胞系中诱导不同的细胞毒性应答，其中CD T阳性分离株显示出显著更高的毒性。内吞途径的阻断表明C.空肠是网格蛋白和动力蛋白依赖性的，但在两种细胞中不依赖于小窝。相反，IL-8（和CXCLi 1/2）的生产依赖于网格蛋白，发动蛋白，和小窝。这项研究是重要的，因为它的规模和产生的数据，表明鸟类和人类上皮细胞使用类似的先天免疫途径，其中响应的幅度是由弯曲杆菌属物种的表型多样性决定的。
Campylobacter remain the major cause of human gastroenteritis in the Developed World causing a significant burden to health services. Campylobacter are pathogens in humans and chickens, although differences in mechanistic understanding are incomplete, in part because phenotypic strain diversity creates inconsistent findings. Here, we took Campylobacter jejuni isolates (n = 100) from multi-locus sequence typed collections to assess their pathogenic diversity, through their inflammatory, cytotoxicity, adhesion, invasion and signaling responses in a high-throughput model using avian and human intestinal epithelial cells. C. jejuni induced IL-8 and CXCLi1/2 in human and avian epithelial cells, respectively, in a MAP kinase-dependent manner. In contrast, IL-10 responses in both cell types were PI 3-kinase/Akt-dependent. C. jejuni strains showed diverse levels of invasion with high invasion dependent on MAP kinase signaling in both cell lines. C. jejuni induced diverse cytotoxic responses in both cell lines with cdt-positive isolates showing significantly higher toxicity. Blockade of endocytic pathways suggested that invasion by C. jejuni was clathrin- and dynamin-dependent but caveolae- independent in both cells. In contrast, IL-8 (and CXCLi1/2) production was dependent on clathrin, dynamin, and caveolae. This study is important because of its scale, and the data produced, suggesting that avian and human epithelial cells use similar innate immune pathways where the magnitude of the response is determined by the phenotypic diversity of the Campylobacter species.
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影响因子：
4.4
作者：
Chaloner, Gemma;Wigley, Paul;Williams, Nicola
通讯作者：
Williams, Nicola
HTRA伴侣的活性有助于弯曲杆菌空肠的宿主细胞结合。
影响因子：
4.2
作者：
Bæk KT;Vegge CS;Brøndsted L
通讯作者：
Brøndsted L
影响因子：
4.8
作者：
ALESSI, DR;CUENDA, A;SALTIEL, AR
通讯作者：
SALTIEL, AR
影响因子：
12.2
作者：
Bennett, William E., Jr.;Gonzalez-Rivera, Rosbel;Tarr, Phillip, I
通讯作者：
Tarr, Phillip, I
影响因子：
3.1
作者：
Hu, L;Bray, MD;Kopecko, DJ
通讯作者：
Kopecko, DJ