Proteomics Profiling of the Urine of Patients with Hyperthyroidism after Anti-Thyroid Treatment.
Proteomics Profiling of the Urine of Patients with Hyperthyroidism after Anti-Thyroid Treatment.
复制标题
DOI：
10.3390/molecules26071991
复制
发表时间：
2021-04-01
期刊：
影响因子：
--
通讯作者：
Alfadda AA
中科院分区：
文献类型：
--
作者：
Benabdelkamel H;Masood A;Ekhzaimy AA;Alfadda AA
甲状腺功能亢进症的特点是循环甲状腺激素水平升高，改变人体的代谢和全身血流动力学平衡，并直接影响肾功能。在这项研究中，使用非靶向蛋白质组学方法和网络分析对甲状腺功能亢进症患者的尿液蛋白质组进行了表征。在卡比马唑治疗前后收集了九名年龄匹配的患者的尿液样本。使用 2D-DIGE 与 MALDI-TOF 质谱联用确定甲状腺功能亢进和甲状腺正常状态之间尿蛋白丰度的差异。通过 Progenesis 软件分析，尿蛋白丰度的变化显示，对应 32 种蛋白的总共 40 个点的丰度存在统计学显着差异，其中 25 个向上，7 个向下（≥1.5 倍变化，方差分析，p ≤ 0.05）。研究中发现的蛋白质可以调节与细胞代谢、运输和急性期反应相关的过程。显着上调的尿蛋白是血清转铁蛋白、运甲状腺素蛋白、血清白蛋白、铜蓝蛋白、α-1B-糖蛋白、syntenin-1 和谷氨酰肽环转移酶，而三种显着下调的蛋白是血浆激肽释放酶、蛋白谷氨酰胺γ-谷氨酰转移酶和丝氨酸蛋白酶抑制剂 B3 (SERPINB3)。使用独创性通路分析 (IPA) 进行的生物信息学分析确定了与细胞损害、炎症反应、细胞组装和组织相关的通路失调，并确定了 APP 和 AKT 信号通路通过与白介素作为中心节点的相互作用而参与其中。
Hyperthyroidism, which is characterized by increased circulating thyroid hormone levels, alters the body’s metabolic and systemic hemodynamic balance and directly influences renal function. In this study, the urinary proteome of patients with hyperthyroidism was characterized using an untargeted proteomic approach with network analysis. Urine samples were collected from nine age-matched patients before and after carbimazole treatment. Differences in the abundance of urinary proteins between hyperthyroid and euthyroid states were determined using a 2D-DIGE coupled to MALDI-TOF mass spectrometry. Alterations in the abundance of urinary proteins, analyzed via Progenesis software, revealed a statistically significant difference in abundance in a total of 40 spots corresponding to 32 proteins, 25 up and 7 down (≥1.5-fold change, ANOVA, p ≤ 0.05). The proteins identified in the study are known to regulate processes associated with cellular metabolism, transport, and acute phase response. The notable upregulated urinary proteins were serotransferrin, transthyretin, serum albumin, ceruloplasmin, alpha-1B-glycoprotein, syntenin-1, and glutaminyl peptide cyclotransferase, whereas the three notable downregulated proteins were plasma kallikrein, protein glutamine gamma-glutamyl transferase, and serpin B3 (SERPINB3). Bioinformatic analysis using ingenuity pathway analysis (IPA) identified the dysregulation of pathways associated with cellular compromise, inflammatory response, cellular assembly, and organization and identified the involvement of the APP and AKT signaling pathways via their interactions with interleukins as the central nodes.
登录
查看更多内容
影响因子：
4.1
作者：
PARRYBILLINGS, M;DIMITRIADIS, GD;NEWSHOLME, EA
通讯作者：
NEWSHOLME, EA
影响因子：
5.3
作者：
Latysheva, Nadya;Muratov, Gairat;Berditchevski, Fedor
通讯作者：
Berditchevski, Fedor
影响因子：
5
作者：
Lunardi, Francesca;Villano, Gianmarco;Calabrese, Fiorella
通讯作者：
Calabrese, Fiorella
影响因子：
4.8
作者：
GREENBERG, CS;BIRCKBICHLER, PJ;RICE, RH
通讯作者：
RICE, RH
影响因子：
4.7
作者：
Nipp, Martin;Elsner, Mareike;Zitzelsberger, Horst
通讯作者：
Zitzelsberger, Horst