Role of Rheum Polysaccharide in the cytokines produced by peripheral blood monocytes in TLR4 mediated HLA-B27 associated AAU.
Role of Rheum Polysaccharide in the cytokines produced by peripheral blood monocytes in TLR4 mediated HLA-B27 associated AAU.
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DOI：
10.1155/2013/431232
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发表时间：
2013
影响因子：
--
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Liu X;Hu X;Zhang X;Li Z;Lu H
目的.目的观察中药大黄多糖（RheumPolysaccharide，RP）对脂多糖（lipopolysaccharide，LPS）刺激的HLA-B27相关性急性前葡萄膜炎恢复期患者单核细胞体外产生肿瘤坏死因子-α（tumornecrosisfactor alpha，TNF-α）和白细胞介素-10（interleukin-10，IL-10）的影响。法从10个HLA-B27相关的急性前葡萄膜炎中分离PBMC样品，分别孵育，并分为以下4组：（1）对照组，加入终浓度为1 mg·L-1的PBS，（2）LPS刺激组，加入终浓度为1 mg·L-1的LPS，（3）LPS + HTA 125刺激组，在加入终浓度为1 mg·L-1的LPS前30分钟，加入终浓度为5 mg·L-1的HTA 125，（4）用LPS + RP刺激，在加入终浓度为1 mg·L-1的LPS前30分钟，加入终浓度为100 mg·L-1的RP。采用酶联免疫吸附试验（ELISA），使用上清液定量随时间推移释放的TNF-α和IL-10的量。结果. LPS刺激后，各时间点患者培养上清中TNF-α和IL-10浓度均显著高于对照组（P < 0.01）。HTA 125阻断TLR-4后，TNF-α和IL-10的产生均较LPS组减少（除IL-10 4 h组外，P < 0.01）。LPS + RP组TNF-α和IL-10水平也明显降低（P < 0.01），但与LPS + HTA 125组相比差异不显著。结论作为抗TLR 4单克隆抗体，大黄多糖还能抑制恢复期HLA-B27阳性AAU患者单核细胞分泌细胞因子。
Purpose. To evaluate the effect of a traditional Chinese medicine, Rheum Polysaccharide (RP), on the in vitro production of tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) by lipopolysaccharide- (LPS-)stimulated human monocytes from HLA-B27 associated acute anterior uveitis patients of convalescence stage. Method. PBMC samples were isolated from 10 HLA-B27 associated acute anterior uveitis, incubated, respectively, and divided into 4 groups as follows: (1) controls, PBS was added in final concentration of 1 mg·L−1, (2) stimulated by LPS, LPS was added in final concentration of 1 mg·L−1, (3) stimulated by LPS + HTA125, 30 minutes before the adding of LPS in final concentration of 1 mg·L−1, the final concentration of 5 mg·L−1 of the HTA125 was added, and (4) stimulated by LPS + RP, 30 minutes before the adding of LPS in final concentration 1 mg·L−1, the final concentration 100 mg·L−1 of the RP was added. Supernatants were used to quantify the amounts of TNF-α and IL-10 released in time course using enzyme-linked immunosorbent assay (ELISA). Result. After stimulated by lps, the concentrations of TNF-α and IL-10 in culture supernatants of patients are significantly higher than control group at all time points (P < 0.01). Blockage of TLR-4 by HTA125 can decrease the production of TNF-α and IL-10 compared with lps group (P < 0.01, except at 4 h group of IL-10). Concentration of TNF-α and IL-10 also decreases in the LPS + RP group (P < 0.01) but not so significantly as in the LPS + HTA125 group. Conclusion. As anti-TLR4 monoclonal antibodies, rheum Polysaccharide can also inhibit the secretion of cytokines produced by monocytes from HLA-B27 positive AAU patients of convalescence stage.
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