Identification of gingipain-specific I-A(b) -restricted CD4+ T cells following mucosal colonization with Porphyromonas gingivalis in C57BL/6 mice.
Identification of gingipain-specific I-A(b) -restricted CD4+ T cells following mucosal colonization with Porphyromonas gingivalis in C57BL/6 mice.
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DOI：
10.1111/omi.12038
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发表时间：
2013-12
影响因子：
3.7
通讯作者：
Costalonga M
中科院分区：
文献类型：
--
作者：
Bittner-Eddy PD;Fischer LA;Costalonga M
Chronic periodontitis is associated with Porphyromonas gingivalis infection. Although virulence factors of P. gingivalis are hypothesized to contribute to the pathogenesis of periodontitis, it is unclear whether the local CD4+ T-cell-mediated response they elicit prevents or contributes to periodontal bone destruction. We hypothesize that major histocompatibility complex class II I-Ab-binding peptides existing in Kgp and RgpA are presented to CD4+ T cells during P. gingivalis oral colonization. The protein sequences of gingipains RgpA and Kgp, and OMP40 and OMP41 of P. gingivalis were scanned using an I-Ab-binding matrix. From this analysis we identified 53 candidate peptides that had the potential to engage the peptide-binding groove of the I-Ab molecule of C57BL/6 mice. An ELISpot-based screen revealed those peptide-primed effector/memory CD4+ T cells that could be re-stimulated in vitro with P. gingivalis or the peptide itself to produce interleukin-17A or interferon-γ. Two immunodominant peptides, Kgp467–477 (pKgp) and RgpA1054–1064/Kgp1074–1084 (pR/Kgp) were identified and engineered to be displayed on I-Ab molecular tetramers. Peptide pR/Kgp is conserved across all sequenced P. gingivalis strains. C57BL/6 mice were orally inoculated with P. gingivalis strain 53977 and cervical lymph node cells were stained with phycoerythrin-conjugated pKgp::I-Ab and pR/Kgp::I-Ab tetramers. We found that only pR/Kgp::I-Ab bound with the desired specificity to gingipain-specific CD4+ T cells. The pR/Kgp::I-Ab tetramer complex will allow the identification of effector/memory CD4+ T cells specific for two virulence factors of P. gingivalis strains associated with periodontal disease.
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影响因子：
3.1
作者：
Baker, PJ;Dixon, M;Roopenian, DC
通讯作者：
Roopenian, DC
影响因子：
--
作者：
Baker, PJ;Dixon, M;Roopenian, DC
通讯作者：
Roopenian, DC
影响因子：
3.1
作者：
Kuboniwa, M;Amano, A;Hamada, S
通讯作者：
Hamada, S
影响因子：
32.4
作者：
Moon, James J.;Chu, H. Hamlet;Jenkins, Marc K.
通讯作者：
Jenkins, Marc K.
DOI：
10.4049/jimmunol.1102661
发表时间：
2012-05-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Jenkins MK;Moon JJ
通讯作者：
Moon JJ