Mechanisms of hearing loss after blast injury to the ear.
Mechanisms of hearing loss after blast injury to the ear.
复制标题
DOI：
10.1371/journal.pone.0067618
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Oghalai JS
中科院分区：
文献类型：
--
作者：
Cho SI;Gao SS;Xia A;Wang R;Salles FT;Raphael PD;Abaya H;Wachtel J;Baek J;Jacobs D;Rasband MN;Oghalai JS
鉴于简易爆炸装置（ied）在世界范围内的频繁使用，对创伤性爆炸伤害的研究日益引起人们的兴趣。耳朵是受爆炸伤害最常见的器官，因为它是人体最敏感的压力传感器。我们制作了一个爆炸室来重建与简易爆炸装置相似的爆炸轮廓，并利用它来建立一个可重复的小鼠模型来研究爆炸引起的听力损失。所有小鼠在爆炸暴露后鼓膜穿孔并发现自愈。显微计算机断层扫描未显示中耳或耳囊损伤的证据；愈合后的鼓膜增厚。听觉脑干反应和畸变产物耳声发射阈值位移与爆炸强度相关。同样，这些阈值的变化比鼓膜穿孔的对照小鼠更大，这表明耳蜗受到了创伤。爆炸后一周和三个月的组织学研究表明，耳蜗内膜没有破坏或损伤。然而，耳蜗基底部的外毛细胞（ohc）减少，螺旋神经节神经元（sgn）和传入神经突触减少。使用我们的小鼠模型来概括人类IED暴露，我们的结果确定爆炸引起的听力损失的机制并不包括通常认为的大体膜破裂。相反，OHC和SGN的损失都会导致听觉功能障碍。
Given the frequent use of improvised explosive devices (IEDs) around the world, the study of traumatic blast injuries is of increasing interest. The ear is the most common organ affected by blast injury because it is the body’s most sensitive pressure transducer. We fabricated a blast chamber to re-create blast profiles similar to that of IEDs and used it to develop a reproducible mouse model to study blast-induced hearing loss. The tympanic membrane was perforated in all mice after blast exposure and found to heal spontaneously. Micro-computed tomography demonstrated no evidence for middle ear or otic capsule injuries; however, the healed tympanic membrane was thickened. Auditory brainstem response and distortion product otoacoustic emission threshold shifts were found to be correlated with blast intensity. As well, these threshold shifts were larger than those found in control mice that underwent surgical perforation of their tympanic membranes, indicating cochlear trauma. Histological studies one week and three months after the blast demonstrated no disruption or damage to the intra-cochlear membranes. However, there was loss of outer hair cells (OHCs) within the basal turn of the cochlea and decreased spiral ganglion neurons (SGNs) and afferent nerve synapses. Using our mouse model that recapitulates human IED exposure, our results identify that the mechanisms underlying blast-induced hearing loss does not include gross membranous rupture as is commonly believed. Instead, there is both OHC and SGN loss that produce auditory dysfunction.
登录
查看更多内容
影响因子：
5.3
作者：
Lang, HN;Schulte, BA;Schmiedt, RA
通讯作者：
Schmiedt, RA
影响因子：
2.8
作者：
HAMERNIK, RP;TURRENTINE, G;WRIGHT, CG
通讯作者：
WRIGHT, CG
影响因子：
2.4
作者：
Ahroon, WA;Hamernik, RP;Lei, SF
通讯作者：
Lei, SF
影响因子：
2.4
作者：
HAMERNIK, RP;PATTERSON, JH;SALVI, RJ
通讯作者：
SALVI, RJ
影响因子：
3.8
作者：
Gao SS;Xia A;Yuan T;Raphael PD;Shelton RL;Applegate BE;Oghalai JS
通讯作者：
Oghalai JS