Transcriptome Analysis of Gene Expression Patterns Potentially Associated with Premature Senescence in Nicotiana tabacum L.
Transcriptome Analysis of Gene Expression Patterns Potentially Associated with Premature Senescence in Nicotiana tabacum L.
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DOI：
10.3390/molecules23112856
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发表时间：
2018-11-02
期刊：
影响因子：
--
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Zhao Z;Li Y;Zhao S;Zhang J;Zhang H;Fu B;He F;Zhao M;Liu P
衰老影响营养物质的再动员和植物对环境的适应。复合胁迫可导致田间植物早衰。本研究通过对成熟叶片和三个不同衰老阶段叶片的转录组学分析，探讨其分子机制。随着衰老进程的进行，叶绿素（chl）含量下降，丙二醛（MDA）含量上升，细胞质和细胞核固缩，线粒体溶解，类囊体片层扩张，叶绿体中淀粉粒减少，嗜锇颗粒增多。此外，还发现了69个在叶片早衰3个阶段共有的差异表达基因（DEG），这些差异表达基因在膜结合细胞器、细胞组分合成调控以及代谢和生物合成过程的基因本体（GO）术语中有显著的富集。京都基因与基因组百科全书（KEGG）途径分析表明，植物激素信号转导途径显著丰富。最后，对常见的DEGs和植物激素信号转导、卟啉和叶绿素代谢、类胡萝卜素生物合成、自噬调控等4条衰老相关途径进行了讨论。本工作旨在为进一步研究提供潜在的基因信号和调控早衰以及可能的基因表达模式的动态网络。
Senescence affects the remobilization of nutrients and adaption of the plant to the environment. Combined stresses can result in premature senescence in plants which exist in the field. In this study, transcriptomic analysis was performed on mature leaves and leaves in three stages of premature senescence to understand the molecular mechanism. With progressive premature senescence, a declining chlorophyll (chl) content and an increasing malonaldehyde (MDA) content were observed, while plasmolysis and cell nucleus pyknosis occurred, mitochondria melted, thylakoid lamellae were dilated, starch grains in chloroplast decreased, and osmiophilic granules increased gradually. Moreover, in total 69 common differentially expressed genes (DEGs) in three stages of premature senescing leaves were found, which were significantly enriched in summarized Gene Ontology (GO) terms of membrane-bounded organelle, regulation of cellular component synthesis and metabolic and biosynthetic processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested that the plant hormone signal transduction pathway was significantly enriched. The common DEGs and four senescence-related pathways, including plant hormone signal transduction, porphyrin and chlorophyll metabolism, carotenoid biosynthesis, and regulation of autophagy were selected to be discussed further. This work aimed to provide potential genes signaling and modulating premature senescence as well as the possible dynamic network of gene expression patterns for further study.
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影响因子：
5.6
作者：
Bita CE;Gerats T
通讯作者：
Gerats T
影响因子：
7.4
作者：
Gan, SS;Amasino, RM
通讯作者：
Amasino, RM
影响因子：
7.2
作者：
Dauwe, Rebecca;Morreel, Kris;Boerjan, Wout
通讯作者：
Boerjan, Wout
影响因子：
4.6
作者：
Duan, Shengchang;Ma, Xiao;Dong, Yang
通讯作者：
Dong, Yang
DOI：
10.1111/tpj.12603
发表时间：
2014-09
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Gallego-Giraldo C;Hu J;Urbez C;Gomez MD;Sun TP;Perez-Amador MA
通讯作者：
Perez-Amador MA