Three-dimensional reconstruction of neovasculature in solid tumors and basement membrane matrix using ex vivo X-ray microcomputed tomography.
Three-dimensional reconstruction of neovasculature in solid tumors and basement membrane matrix using ex vivo X-ray microcomputed tomography.
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DOI：
10.1111/micc.12102
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Goldfinger LE
中科院分区：
文献类型：
--
作者：
Lee S;Barbe MF;Scalia R;Goldfinger LE
在异种移植肿瘤和基质胶栓塞中创建准确、高分辨率的新生血管结构3D重建，用于动物模型血管生成研究中的定量分析。小鼠异种移植实体瘤或Matrigel栓塞内的活性新血管系统通过心脏全身灌注血液循环，用Microfil（一种不透射线的亲水性聚合造影剂）灌注。切除灌注的肿瘤和栓塞，并通过X射线显微计算机断层扫描生成显示不透射线材料的2D图像堆栈。采用无偏、精确的后处理方案消除血管外组织的背景X射线吸收。编辑修订后的二进制图像堆栈，以显示3D重建的Microfil-cast新生血管。使用扫描仪软件从精细的3D重建计算血管结构参数。澄清的3D重建足够精确，可以测量肿瘤和栓塞中的血管结构，分辨率的直径限制为3 μm。离体显微计算机断层扫描可用于三维重建和定量分析的新血管，包括微循环的实体肿瘤和基质胶塞。该方法可广泛应用于三维血管结构的重建和测量。
To create accurate, high resolution 3D reconstructions of neovasculature structures in xenografted tumors and Matrigel plugs for quantitative analyses in angiogenesis studies in animal models. The competent neovasculature within xenografted solid tumors or Matrigel plugs in mice was perfused with Microfil, a radio-opaque, hydrophilic polymerizing contrast agent, by systemic perfusion of the blood circulation via the heart. The perfused tumors and plugs were resected and scanned by X-ray micro-computed tomography to generate stacks of 2D images showing the radio-opaque material. A non-biased, precise post-processing scheme was employed to eliminate background X-ray absorbance from the extra-vascular tissue. The revised binary image stacks were compiled to reveal the Microfil-casted neovasculature as 3D reconstructions. Vascular structural parameters were calculated from the refined 3D reconstructions using the scanner software. Clarified 3D reconstructions were sufficiently precise to allow measurements of vascular architecture to a diametric limit of resolution of 3 μm in tumors and plugs. Ex vivo micro-computed tomography can be used for 3D reconstruction and quantitative analysis of neovasculature including microcirculation in solid tumors and Matrigel plugs. This method can be generally applied for reconstructing and measuring vascular structures in 3 dimensions.
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影响因子：
3.7
作者：
Downey, Charlene M.;Singla, Arvind K.;Jirik, Frank R.
通讯作者：
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影响因子：
4
作者：
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影响因子：
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影响因子：
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作者：
Lee S;Wurtzel JG;Singhal SS;Awasthi S;Goldfinger LE
通讯作者：
Goldfinger LE
影响因子：
2.5
作者：
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通讯作者：
Djonov, Valentin