Transcriptional analysis reveals formation of axillary solitary hook in vine plant Uncaria rhynchophylla
Transcriptional analysis reveals formation of axillary solitary hook in vine plant Uncaria rhynchophylla
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转录分析揭示藤本植物钩藤腋生单钩的形成
DOI：
10.1007/s11816-022-00808-3
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发表时间：
2022-12
影响因子：
2.4
通讯作者：
Zhanjiang Zhang
中科院分区：
文献类型：
--
作者：
Lingyun Wan;Limei Pan;Lisha Song;Jine Fu;Qiulan Huang;Xiaowen Ji;Shugen Wei;Zhanjiang Zhang
摘要 钩藤钩藤是代表钩藤品质的重要外在表型。钩藤的钩形成模式具有独特的特征，即单钩和对生钩交替出现在叶腋中，形成衬系列。单生钩仅生长在叶腋下侧，是钩总产量下降的结果。然而，钩藤形成孤钩的原因尚不清楚。因此，迫切需要对孤钩形成的分子机制进行研究。我们从腋窝茎段下侧（HS）和腋窝茎段上侧（HSCK）文库获得了 25067 万条高质量的干净读数。我们在HS和HSCK文库之间检测到42个DEG（差异表达基因）；下调11个，上调31个。 DEGs的基因本体功能分类表明，它们包括两个编码与极性生长素运输调节相关的LAZY1的基因，两个编码与植物激素信号转导相关的AUX/IAA和AP2/ERF的基因，以及两个编码与黄酮合酶活性相关的F3H的基因。这六个基因与侧极生长素转运（PAT）调节相关，它们的表达水平与重力刺激下IAA的分布模式具有高度相关性。用N-1-萘基邻苯二甲酸（NPA）或槲皮素（被称为极性生长素抑制剂）处理后，观察到单钩发育受到抑制。上述研究结果可能为重力作用下腋芽发育过程中侧向PAT调控的分子机制提供新的信息。
AbstractThe hook of Uncaria rhynchophylla is an important external phenotype representing the quality of Gou-Teng. The hook formation pattern of U. rhynchophylla displays a unique feature that solitary hook and opposite hooks alternately occur in the leaf axils forming a liner series. The solitary hook only grows on the lower side of the leaf axil, resulting from the decline in the total yield of hooks. However, the reasons for solitary hook formation in U. rhynchophylla are not clear. Therefore, a survey on the molecular mechanisms of solitary hook formation needs to be performed urgently. We obtained 250.67 million high-quality clean reads from the lower side of axillary stem segments (HS) and the upper side of axillary stem segments (HSCK) libraries. We detected 42 DEGs (differentially expressed genes) between HS and HSCK libraries; 11 were down-regulated, and 31 were up-regulated. Gene ontology functional classification of the DEGs indicated that they included two genes that encoded LAZY1 related to the regulation of polar auxin transport, two genes that encoded AUX/IAA and AP2/ERF related to plant hormone signaling transduction, and two genes that encoded F3H related to the flavone synthase activity. These six genes were associated with lateral polar auxin transport (PAT) regulation, and their expression level had a high correlation with the pattern of IAA distribution upon gravity stimulation. And the inhibition of solitary hook development was observed after treatment with N-1-naphthylphthalamic acid (NPA) or quercetin, which was known as the polar auxin inhibitor. The above findings might provide new information about the molecular mechanisms of regulation of lateral PAT in the axillary bud development process under gravity.
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影响因子：
6.1
作者：
KURAMOCHI, T;CHU, J;SUGA, T
通讯作者：
SUGA, T
DOI：
10.2307/25528250
发表时间：
2021-01
期刊：
The Fundamentals of Creative Photography
影响因子：
--
作者：
David Präkel
通讯作者：
David Präkel
DOI：
10.1073/pnas.1717649115
发表时间：
2018-04-24
影响因子：
11.1
作者：
Vaseva II;Qudeimat E;Potuschak T;Du Y;Genschik P;Vandenbussche F;Van Der Straeten D
通讯作者：
Van Der Straeten D
综合转录组分析揭示了茶树腋芽在旁休眠、内休眠、生态休眠和芽盛期的差异基因表达谱
影响因子：
5.6
作者：
Hao X;Yang Y;Yue C;Wang L;Horvath DP;Wang X
通讯作者：
Wang X
影响因子：
7.4
作者：
J. S. Lee;Chang W-K;M. Evans
通讯作者：
J. S. Lee;Chang W-K;M. Evans