In silico and Genetic Analyses of Cyclic Lipopeptide Synthetic Gene Clusters in Pseudomonas sp. 11K1
In silico and Genetic Analyses of Cyclic Lipopeptide Synthetic Gene Clusters in Pseudomonas sp. 11K1
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假单胞菌环状脂肽合成基因簇的计算机模拟和遗传分析。
DOI：
10.3389/fmicb.2019.00544
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发表时间：
2019-03
影响因子：
5.2
通讯作者：
Zhang Li Qun
中科院分区：
文献类型：
--
作者：
Zhao Hui;Liu Yan Ping;Zhang Li Qun
假单胞菌属11K1最初是从根际分离出来的，对植物病原真菌和细菌具有抑制活性。在此，我们对11K1菌株的基因组进行了测序，并对其进行了电子、突变和功能分析。11K1基因组全长6,704,877个碱基，基因组挖掘发现了3个潜在的环脂肽(CLP)生物合成簇，分别命名为Brasmycin、brasPeptin和brasamine。插入突变体和缺失突变体的抗菌活性下降，但对水稻白叶枯病菌的抗菌活性保持不变。根据腺化(A)结构域预测了这两个活性CLP的结构。布拉斯霉素由9个氨基酸组成，属于丁香霉素类，而布拉斯肽是一种22个氨基酸的环肽，属于托拉菌素类。基质辅助激光解吸/电离飞行时间(MALDI-TOF)质谱仪分析表明，与已知的丁香霉素和托拉菌素相比，布拉斯霉素和布拉斯汀具有不同的相对分子质量。基因簇突变对生物膜的形成和菌落形态均有影响。综上所述，这些结果表明，假单胞菌属。11K1产生两种新的CLP，可能有助于细菌在环境中竞争营养和生态位。
Pseudomonas sp. 11K1, originally isolated from rhizosphere, possesses inhibitory activity against plant pathogenic fungi and bacteria. Herein, the genome of strain 11K1 was sequenced and subjected to in silico, mutational, and functional analyses. The 11K1 genome is 6,704,877 bp in length, and genome mining identified three potential cyclic lipopeptide (CLP) biosynthetic clusters, subsequently named brasmycin, braspeptin, and brasamide. Insertional and deletion mutants displayed impaired brasmycin and braspeptin production, and lost antifungal activity, but retained antibacterial activity against Xanthomonas oryzae. The structures of these two active CLPs were predicted based on adenylation (A) domains. Brasmycin is composed of nine amino acids and belongs to the syringomycin class, while braspeptin is a 22 amino acid cyclic peptide belonging to the tolaasin group. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry analysis revealed that brasmycin and braspeptin have different molecular weights compared with known syringomycin and tolaasin members, respectively. Mutation of brasmycin and braspeptin gene clusters affected both biofilm formation and colony morphology. Collectively, these results indicate that Pseudomonas sp. 11K1 produces two novel CLPs that may help bacteria compete for nutrients and niches in the environment.
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对假单胞菌特异代谢组进行索引使得聚酰胺 B 和香蕉酰胺的发现成为可能。
影响因子：
28.3
作者：
Nguyen DD;Melnik AV;Koyama N;Lu X;Schorn M;Fang J;Aguinaldo K;Lincecum TL Jr;Ghequire MG;Carrion VJ;Cheng TL;Duggan BM;Malone JG;Mauchline TH;Sanchez LM;Kilpatrick AM;Raaijmakers JM;De Mot R;Moore BS;Medema MH;Dorrestein PC
通讯作者：
Dorrestein PC
影响因子：
3.2
作者：
D. Weller
通讯作者：
D. Weller
影响因子：
2.7
作者：
Lavermicocca, P;Iacobellis, NS;Graniti, A
通讯作者：
Graniti, A
影响因子：
4.9
作者：
C. Strano;P. Bella;G. Licciardello;A. Fiore;A. R. Lo Piero;V. Fogliano;V. Venturi;V. Catara
通讯作者：
C. Strano;P. Bella;G. Licciardello;A. Fiore;A. R. Lo Piero;V. Fogliano;V. Venturi;V. Catara
影响因子：
3.2
作者：
A. Toguchi;Michael A. Siano;M. Burkart;R. Harshey
通讯作者：
A. Toguchi;Michael A. Siano;M. Burkart;R. Harshey