A comparative study of “turn-off” mode and “turn-on” mode lateral flow immunoassay for T-2 toxin detection
A comparative study of “turn-off” mode and “turn-on” mode lateral flow immunoassay for T-2 toxin detection
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T-2毒素“关闭”模式与“开启”模式侧流免疫分析的比较研究
DOI：
10.1016/j.snb.2022.131545
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发表时间：
2022-02
期刊：
影响因子：
--
通讯作者：
Meng Dang
中科院分区：
文献类型：
--
作者：
Xiya Zhang;Mingyue Ding;Yexuan Mao;Xianqing Huang;Xinhua Xie;Lianjun Song;Mingwu Qiao;Jianwei Zhang;Tianlin Wang;Haihua Zhu;Zizhe Li;Youyi Wang;Meng Dang
以T-2毒素为模型分子，以T-2-BSA和抗T-2单克隆抗体为免疫复合物对，对基于传统竞争模式的“关闭”模式侧流免疫分析（LFA）和基于内过滤效应的“开启”模式侧流免疫分析（IFE-LFA）进行了客观比较，以提高LFA的灵敏度。首先，选择Au纳米颗粒（Au NPs）、无定形碳纳米颗粒（ACNPs）、量子点纳米球（QD）和时间分辨荧光微球（TRFMs）作为用于开发“关断”模式LFA（分别为Au NPs-LFA、ACNPs-LFA、QDs-LFA和TRFMs-LFA）的标记物。然后，以Au NPs和ACNPs为吸收剂，以量子点和TRFMs为荧光剂，制备了“开启”模式的IFE-LFAs（基于Au NPs作为吸收剂和QD作为荧光剂的IFE-LFA [Au NPs-mAb-QDs-BSA]，基于Au NPs作为吸收剂和TRFMs作为荧光剂的IFE-LFA [Au NPs-mAb-TRFms-BSA]，基于ACNP作为吸收剂和QD作为荧光剂的IFE-LFA [ACNPs-mAb-QDs-BSA]，和基于ACNP作为吸收剂和TRFM作为荧光剂的IFE-LFA [ACNPs-mAb-TRFMs-BSA]）。在优化条件下，上述8种LFA的肉眼观察截止值分别为4、2、2、2、3、2、1.5和1 ng/mL，定量检测限分别为0.50、0.23、0.24、0.23、0.47、0.45、0.27和0.22 ng/mL。在所有LFA中，ACNPs-mAb-TRFMs-BSA显示出最高的灵敏度。该研究结果为提高低灵敏度荧光分析仪的灵敏度以满足现场痕量物质筛查的要求提供了参考。
The “turn-off” mode lateral flow immunoassay (LFA) based on the conventional competitive format and “turn-on” mode LFA mainly determined by inner filter effect (IFE-LFA) were objectively compared using T-2 toxin as a model molecule and T-2-BSA and anti-T-2 monoclonal antibody as immunocomplex pairs to improve the sensitivity of the LFA. First, Au nanoparticles (Au NPs), amorphous carbon nanoparticles (ACNPs), quantum dots nanospheres (QDs), and time-resolved fluorescent microspheres (TRFMs) were selected as labels for developing the “turn-off” mode LFAs (Au NPs-LFA, ACNPs-LFA, QDs-LFA, and TRFMs-LFA, respectively). Thereafter, Au NPs and ACNPs were used as absorbers, and QDs and TRFMs were selected as fluorescers, for preparing the “turn-on” mode IFE-LFAs (IFE-LFA based on Au NPs as absorbers and QDs as fluorescers [Au NPs-mAb-QDs-BSA], IFE-LFA based on Au NPs as absorbers and TRFMs as fluorescers [Au NPs-mAb-TRFMs-BSA], IFE-LFA based on ACNPs as absorbers and QDs as fluorescers [ACNPs-mAb-QDs-BSA], and IFE-LFA based on ACNPs as absorbers and TRFMs as fluorescers [ACNPs-mAb-TRFMs-BSA]). Under optimized conditions, the naked-eye observation cut-off values for the aforementioned eight LFAs were 4, 2, 2, 2, 3, 2, 1.5, and 1 ng/mL, with quantitative limit of detection values of 0.50, 0.23, 0.24, 0.23, 0.47, 0.45, 0.27, and 0.22 ng/mL, respectively. Among all LFAs, ACNPs-mAb-TRFMs-BSA showed the highest sensitivity. The results of this study provide a perspective for improving the sensitivity of the LFAs to meet the requirements of on-site screening for trace substances.
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影响因子：
4.1
作者：
Huang, Chao;Wen, Tian;Jiao, Yong-Jun
通讯作者：
Jiao, Yong-Jun
DOI：
10.1016/j.jphotochem.2007.04.032
发表时间：
2007-11
影响因子：
4.3
作者：
Cong-Xia Wang;Fangfei Yan;Yixuan Zhang;Ling Ye
通讯作者：
Cong-Xia Wang;Fangfei Yan;Yixuan Zhang;Ling Ye
影响因子：
6.1
作者：
Zhang, Xiya;Yu, Xuezhi;Wang, Zhanhui
通讯作者：
Wang, Zhanhui
影响因子：
5.7
作者：
Anfossi, Laura;Di Nardo, Fabio;Baggiani, Claudio
通讯作者：
Baggiani, Claudio
影响因子：
12.6
作者：
Liu, Bing;Wang, Lingling;Wang, Shuo
通讯作者：
Wang, Shuo