Using chondroitin sulfate to improve the viability and biosynthesis of chondrocytes encapsulated in interpenetrating network (IPN) hydrogels of agarose and poly(ethylene glycol) diacrylate.
Using chondroitin sulfate to improve the viability and biosynthesis of chondrocytes encapsulated in interpenetrating network (IPN) hydrogels of agarose and poly(ethylene glycol) diacrylate.
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DOI：
10.1007/s10856-011-4499-9
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发表时间：
2012-01
影响因子：
3.7
通讯作者：
Detamore, Michael S.
中科院分区：
文献类型：
--
作者：
Ingavle, Ganesh C.;Dormer, Nathan H.;Gehrke, Stevin H.;Detamore, Michael S.
我们最近将琼脂-聚乙二醇二丙烯酸酯(PEGDA)互穿网络(IPN)水凝胶引入到软骨组织工程中，这种水凝胶能够包裹活细胞，并相对于其两种组成的水凝胶提供了显著的机械性能改善。本研究的目的是开发一种新的合成方案，将甲基丙烯酸化的硫酸软骨素(MCS)引入到假想的IPN设计中，以提高细胞活力和生物合成。将猪软骨细胞包裹在琼脂糖凝胶中，将其浸泡在1：10MCS：PEGDA溶液中，然后进行光聚合，形成共聚网络作为第二网络。含有CS(CS-IPN)(~0.5wt%)的IPN相对于PEGDA或琼脂糖凝胶的压缩弹性模量增加了4~5倍。体外培养6周后，包裹的软骨细胞在CS修饰的IPN中仍有50%以上的存活，而在未修饰的IPN中观察到35%的存活。在第6周，CS-IPN的标准化GAG含量(347±34微克/微克)显著高于未改良的IPN(158±27微克/微克，P<；0.05)。总体而言，将天然组织中的CS等生物聚合物掺入细胞的方法可能会为细胞提供有利的微环境和有益的信号，以提高其在IPN中的整体性能。
We recently introduced agarose-poly(ethylene glycol) diacrylate (PEGDA) interpenetrating network (IPN) hydrogels to cartilage tissue engineering that were able to encapsulate viable cells and provide a significant improvement in mechanical performance relative to its two constituent hydrogels. The goal of the current study was to develop a novel synthesis protocol to incorporate methacrylated chondroitin sulfate (MCS) into the IPN design hypothesized to improve cell viability and biosynthesis. The IPN was formed by encapsulating porcine chondrocytes in agarose, soaking the construct in a solution of 1:10 MCS:PEGDA, which was then photopolymerized to form a copolymer network as the second network. The IPN with incorporated CS (CS-IPN) (~0.5 wt%) resulted in a 4- to 5-fold increase in the compressive elastic modulus relative to either the PEGDA or agarose gels. After 6 weeks of in vitro culture, more than 50% of the encapsulated chondrocytes remained viable within the CS-modified IPN, in contrast to 35% viability observed in the unmodified. At week 6, the CS-IPN had significantly higher normalized GAG contents (347 ± 34 µg/µg) than unmodified IPNs (158 ± 27 µg/µg, P < 0.05). Overall, the approach of incorporating biopolymers such as CS from native tissue may provide favorable micro-environment and beneficial signals to cells to enhance their overall performance in IPNs.
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DOI：
10.1115/1.1318906
发表时间：
2000-12-01
影响因子：
1.7
作者：
Butler, DL;Goldstein, SA;Guilak, F
通讯作者：
Guilak, F
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
DOI：
10.1097/00003086-200110001-00009
发表时间：
2001-10-01
影响因子：
4.2
作者：
D'Lima, DD;Hashimoto, S;Lotz, MK
通讯作者：
Lotz, MK
影响因子：
5.5
作者：
Li, Q;Wang, DA;Elisseeff, JH
通讯作者：
Elisseeff, JH
影响因子：
14
作者：
Kutty, Jaishankar K.;Cho, Eunhee;Webb, Ken
通讯作者：
Webb, Ken