Lipid domain formation and non-lamellar structures associated with varied lysylphosphatidylglycerol analogue content in a model Staphylococcal plasma membrane.
Lipid domain formation and non-lamellar structures associated with varied lysylphosphatidylglycerol analogue content in a model Staphylococcal plasma membrane.
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脂质结构域的形成和非层状结构与模型葡萄球菌质膜中不同的赖氨酰磷脂酰甘油类似物含量相关。
DOI：
10.1016/j.bbamem.2021.183571
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Rehal R
中科院分区：
文献类型：
--
作者：
Rehal R
二棕榈酰-3-氮杂-脱羟基-赖氨酰磷脂酰甘油（DP 3adLPG）是细菌脂质赖氨酰磷脂酰甘油（LPG）的化学稳定的合成类似物，在生物物理研究中被设计为众所周知的不稳定天然脂质的替代物。在金黄色葡萄球菌中，已知LPG通过改变膜电荷特性以响应环境应激而在抗生素抗性中发挥作用，但关于LPG如何通过与脂质如磷脂酰甘油（PG）形成复合物影响其他双层理化性质或横向组织知之甚少。在这项研究中，我们已经调查了不同的相形成的仿生混合物的3adLPG和PG在不同的热致状态，使用中子衍射和电子显微镜。在DPPG/DP 3adLPG 70：30 mol%混合物中，在脂质熔融转变下方观察到两个不同的层状相：Lβ′1和Lβ′2，其周期分别为82和62 π。增加DP 3adLPG的比例以模拟环境应力的影响，导致Lβ′1相消失并形成反六方相。通过研究两种混合物的热致性，并探测它们与抗菌肽magainin 2 F5 W的相互作用，确定了这些不同相的组成。我们建议，所观察到的多态性的结果从优先形成的三重PG-3adLPG-PG，或配对的PG-3adLPG复合物，取决于两种脂质的混合比例。这些研究结果的相关性的作用天然液化石油气inS. aureus，讨论了其对抗生素耐药性和侧膜组织的影响。
Dipalmitoyl-3-aza-dehydroxy-lysylphosphatidylglycerol (DP3adLPG), is a chemically stable synthetic analogue of the bacterial lipid lysylphosphatidylglycerol (LPG), designed as a substitute for the notoriously labile native lipid in biophysical investigations. InStaphylococcus aureus, LPG is known to play a role in resistance to antibiotics by altering membrane charge properties in response to environmental stress, but little is known about how LPG influences other bilayer physicochemical properties or lateral organisation, through the formation of complexes with lipids such as phosphatidylglycerol (PG). In this study we have investigated the different phases formed by biomimetic mixtures of 3adLPG and PG in different thermotropic states, using neutron diffraction and electron microscopy. In a DPPG/DP3adLPG 70:30 mol% mixture, two distinct lamellar phases were observed below the lipid melting transition: Lβ′1 and Lβ′2 with respective periodicities of 82 and 62 Å. Increasing the proportion of DP3adLPG to mimic the effects of environmental stress led to the disappearance of the Lβ′1 phase and the formation of an inverse hexagonal phase. The compositions of these different phases were identified by investigating the thermotropic properties of the two mixtures, and probing their interaction with the antimicrobial peptide magainin 2 F5W. We propose that the observed polymorphism results from the preferential formation of either triplet PG-3adLPG-PG, or paired PG-3adLPG complexes, dependent upon the mixing proportions of the two lipids. The relevance of these findings to the role native LPG inS. aureus, are discussed with respect to their influence on antibiotic resistance and lateral membrane organisation.
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呼吸金黄色葡萄球菌和大肠杆菌的质子动力大小
影响因子：
3.2
作者：
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影响因子：
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作者：
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通讯作者：
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